中文摘要
分子标记辅助育种平台的建立是甘蔗分子育种研究的难点,也是未来广西甘蔗育种工作的重点。广西是我国甘蔗最大的生产基地和产糖中心。新品种选育成为解决甘蔗品种单一、抗逆性差以及抵御自然风险最有效的措施之一。近年来,甘蔗亲本种质资源遗传基础狭窄和野生种血缘减少,已成为常规育种取得重大突破的重要限制因素。随着分子标记技术在甘蔗辅助育种上的发展和应用,甘蔗分子育种研究也进入以现代生物工程为主要特征的育种新阶段。本项目将应用SNP技术发掘更多与甘蔗重要农艺性状(即脱叶性)相连锁的SNPs位点。项目组已筛选出12个易脱叶亲本和6个难脱叶亲本,并配成30个常规杂交组合,利用甘蔗"五圃制"育种技术分阶段筛选F1分离群体。本项目拟通过SNP技术获得与甘蔗脱叶性相连锁的SNP位点,应用生物信息学技术分析甘蔗种质与脱叶性状的遗传关联性。
英文摘要
The establishment of platform for molecular marker-assisted selection is one of the difficult in sugarcane molecular breeding. And it is one of the emphases in sugarcane breeding in Guangxi province. Guangxi has become the largest producer of sugarcane, and the center of cane sugar producing in China. Selective breeding of new sugarcane varieties has become one of the most effective measures to solve the problems of single species, bad resistance to adversity, and the weak of capability for defense against natural disasters. Recently, the sugarcane varieties bred in China are limited in the narrowed hereditary basis of sugarcane germplasm resource and the diminution of the blood of wild sugarcane germplasm. With the development and application of molecular markers rapidly in sugarcane assisted selection, the research of sugarcane molecular breeding has entered a new breeding stage characterized by modern biotechnology. In this project, screening genetic markers of important agronomic traits (such as deciduous leaf) of sugarcane by using single nucleotide polymorphism (SNP). Total eighteen sugarcane germplasm resources were selected, twelve germplasm resources were easy deciduous, and six germplasm resources were holds onto its leaves. All the germplasm resources were studied by using 30 combinations. Sugarcane germplasm resources and F1 segregation population of all combinations were selected by using sugarcane breeding program five nursery systems. In this project, more and more SNPs linked tightly to important agronomic traits (such as deciduous leaf) will be acquired and obtained, and the objectives are to enhance the capability manipulating SNPs molecular markers assisted-selected breeding for breeders, and provide theory basis for further important agronomic traits SNPs screening, cloning and application.
