申请人近年专注运动创伤修复研究，发表成系列肌腱修复相关SCI论文26篇（平均影响因子5.86，SCI他引425次，单篇最高98次，H指数13）。其中第一/通讯作者7篇，共同第一/通讯7篇(平均影响因子6.23).主要创新: 1.创新肌腱分化模型并发现分化新机制：在国际上率先报道全能干细胞的肌腱分化体系，证明胚胎来源细胞是肌腱再生的理想种子细胞，建立无支架组织工程肌腱模型用于研究肌腱分化。设计转录因子与力学刺激程序式调控肌腱分化的方案并解释机制。2. 研发功能性肌腱支架：创新采用网状结构设计一个兼具有优良力学功能和足够内在空隙的功能性肌腱蚕丝支架。研究成果2010年获国际肌腱韧带专题会议YIA，2014年教育部自然科学二等奖.本项目拟在前期研究基础上，根据肌腱发育阶段和干细胞分化时序基因调控及组织工程等手段探索肌腱发育不同阶段的分化调控因子。以实现干细胞向肌腱分化的阶段性诱导手段及机理

The applicant has committed himself to scientific research for a long time in the field of sports system injury and regeneration. He has published a series of tendon related papers (26 total, average Impact factor is approximately 5.86, 425 times are cited by others, highest 98 times, H index 13). 7 of which are first or corresponding author papers, while 7 of which are co-first or co-corresponding author papers (average Impact factor is approximately 6.23). .His major achievements are: 1. Found a new tendon differentiation model and recover new mechanism of tendon differentiation: we first report that we induced the pluripotent stem cells into tenocytes, proved that embryonic cells are more suitable for tendon tissue engineering, established in vitro scaffold-free tendon tissue engineering model, found that transcription factor and mechanical stress have synergetic function on tendon differentiation ; 2. Fabricated functional tendon scaffold: we used knitted silk scaffold with proper mechanical strength and internal spaces for tissue growth. Based on these work, he gained YIA of the international tendon/ligament conference in 2010. He also gained the second prize of the Natural Science Award of ministry of education..Based on these earlier work, this project will explore the mechanisms of tendon differentiation stage , as well as the stage specific tendon differentiation induction methods, according to the tendon development stage and sequential gene expression profile during stem cells differentiation.