广西是我国甘蔗主产区，然而蔗地90%以上为旱坡地，土壤保水保肥能力差，给生产带来巨大损失，充分挖掘自然生态系统中共生微生物的促生和抗逆潜力，可提高我国甘蔗生产能力、保护和改善蔗地生态环境。在前期研究中,我们从南宁市郊甘蔗根围分离获得2株能显著促进甘蔗生长的DSE菌株，发现DSE在蔗地生态系统中可能扮演着较为重要的生态角色。为了进一步全面发掘甘蔗根围DSE资源，本研究拟对广西主蔗区甘蔗根围的DSE物种展开系统的调查研究，采用茄科植物诱捕分离根围土壤的DSE菌株，并结合形态学和核糖体DNA的ITS 、SSU和LSU序列分析对其进行分类鉴定，通过建立"DSE-甘蔗"共生体，探讨DSE对甘蔗生长、抗旱能力的影响，目的在于明确广西甘蔗根围DSE的物种多样性与分布、筛选到对甘蔗具显著促生和提高抗旱能力的菌株，为探明DSE在自然生态系统中的作用、利用其促进甘蔗生产的节肥减耗及蔗地生态的良性循环提供依据。

Guangxi is the sugarcane main production area in China, however, 90% sugarcane are planted in arid hillside field and hard to conserve water and fertilizer which caused tremendous production loss. Give full play to the potential of symbiotic microorganism's plant growth promotion and adverse resistance functions in nature ecology system can improve sugarcane production and conservation of sugarcane filed ecosystem. we had obtained two DSE isolates from sugarcane rhizosphere in the suburb of Nanning city which could promote sugarcane growth significantly, this suggested that DSE may play a important ecological role in sugarcane filed ecosystem. To full excavate the DSE resource from sugarcane rhizosphere, in this research, we will study the DSE species diversity in sugarcane rhizosphere, isolate the DSE fungi in rhizosphere soil of different sugarcane habitat by using egg plant as bating plant, and identify DSE species according to the morphology and the sequence analysis of the ITS ,SSU and LSU regions of rDNA, and screen for the effected isolate by study the effect of DSE fungi on the growth promotion and drought-resistant ability of sugarcane.The objective of this study is to clear understanding of the DSE species diversity and distribution in sugarcane rhizosphere and obtain the ecological functional DSE strain, this result has good promote to study of DSE ecological function in natural ecosystem, and will provide theoretic basis to use DSE biotechnology for raising sugarcane production capacity and conservation of sugarcane filed ecosystem.