大蒜素能够有效干预金黄色葡萄球菌生物被膜的形成，其机制可能是通过作用sarA/agr双重信号通路实现的。本研究以不同地域、不同类型奶牛乳房炎源性金黄色葡萄球菌分离株为研究对象，通过质粒重组构建sarA基因突变株、agr基因突变株和sarA/agr双基因突变株，应用扫描电镜（SEM）和共聚焦激光扫描电镜（CLSM）研究大蒜素对生物被膜形态的影响，应用结晶紫法研究生物被膜初始黏附与分离、化学发光免疫法检测PIA产量、XTT法和荧光发光法检测胞内菌的数量与活力、RT-PCR和Western Bloting等检测基因表达、ELISA法检测毒力因子的产量，最后通过数据分析建立数学模型。本研究旨在揭示sarA/agr双重介导下的大蒜素抗金黄色葡萄球菌生物被膜机制，并在此基础上阐明大蒜素对毒力因子和耐药性的影响规律，为金黄色葡萄球菌生物被膜相关感染的控制提供理论依据。

Allicin can effectively interfere with biofilm formation of Staphylococcus aureus whose mechanism may be performed by sarA/agr dual mediated signaling pathway. In this study, Staphylococcus aureus isolates were taken as object from different types of bovine mastitis in different regions, constructing sarA mutant, agr mutant, and sarA/agr double mutant by recombinant plasmid, studying the effect of allicin on biofilm morphology by scaning electron microscope (SEM) and confocal laser scanning microscope (CLSM), studying initial adherent and detachment of biofilm by crystal violet staining method, detecting PIA production by Chemiluminescence immunoassay, decting the number and vitality of intracellular bacteria by XTT and Fluorescent luminescence, detecting gene expressions by RT-PCR and Western Blotting, detecting virulence factors by ELISA, finally, establishing mathematical model by data analysis. This study aims to reveal the mechanism of allicin against biofilm of Staphylococcus aureus in sarA/agr dual mediation, and on the basis of the mechanism clarifies the influence of allicin on virulence factors and drug resistance, which can provide a theoretical basis to control Staphylococcus aureus biofilm-associated infections.