蛋鸡脂肪肝出血综合征(Fatty liver hemorrhagic syndrome，FLHS)是蛋鸡体内脂肪代谢紊乱引起的以肝脏发生脂肪变性、出血为特征的一营养代谢性疾病，是高产蛋鸡常见多发病。临床上发病原因主要涉及营养因素、遗传因素、管理因素、环境因素、激素以及有毒物质等，其中，高能低蛋白日粮是我国目前生产上蛋鸡发生脂肪肝出血综合征的主要原因，但其发病机制并不清楚。AMPK及其信号通路与动物机体脂肪代谢密切相关。因此，本研究利用原位杂交、荧光定量PCR、Western blotting、免疫组化、计算机图像分析技术和临床病理学等检测技术，体内和体外试验相结合，研究AMPK及其信号通路与患FLHS蛋鸡体内脂肪代谢的相关性，从而揭示AMPK及其信号通路与高能低蛋白日粮致FLHS的关系，进而部分阐明高能低蛋白日粮致FLHS的机理，为高产蛋鸡FLHS的防治提供理论基础。

Fat liver hemorrhage syndrome (FLHS) is a nutritional and metabolic disease caused by fat metabolism disorder and characterized by fatty degeneration and bleeding of the liver. It is a common disease in high yield hens. Clinical causes mainly related to nutritional factors, genetic factors, management factors, environmental factors, hormones and toxic substances. High-energy low-protein diet is the main cause of fatty liver haemorrhagic syndrome on the producing laying hens, but the pathogenesis is still not clear. AMPK (AMP-activated protein kinase) and its network are closely related to the animal body fat metabolism. Therefore, this study uses in situ hybridization, real-time PCR, Western blotting, immunohistochemistry and computer image analysis techniques and clinical pathology detection technology to study the role of AMPK pathway, in vivo and in vitro, in FLHS laying hens fat metabolism, and to reveal the relationship of AMPK pathway with FLHS caused by high-energy low-protein diets, and partially elucidate the potential mechanism, provide a theoretical basis for the prevention and treatment of FLHS in high-yielding hens.