经典方剂是中医治疗疾病的主要手段，其不仅通过多成分、多靶点直接调节靶器官分子网络，也经由神经内分泌间接调节人体网络。本研究以补益肝肾经方二至丸为研究对象，在明确其化学物质、雌雄激素活性及临床疗效工作基础上，围绕 “二至丸类雌雄激素样作用途径是其补益肝肾的生物学基础”假说。采用雌雄性激素直接或间接经由神经内分泌作用的靶器官肾上腺、前列腺、乳腺、骨、脑、肝脏等相关细胞，研究二至丸入血化学成分雌雄激素基因组和非基因组效应；采用在体类似肝肾阴虚证的自然快速老化的SAMP6骨松小鼠、SAMP8痴呆小鼠、去势大鼠以及基因（AR、ER、LDLR、ApoE）敲除小鼠等动物模型，研究二至丸补益肝肾的生物学基础；选择肝肾阴虚型围绝经期高血压或骨质疏松症受试者验证其科学性。整合基础和临床研究结果阐明二至丸治疗肝肾阴虚疾病的生物学基础，提高其治疗慢性病的精准性，也为中医“异病同治”提供科学依据。

The Classical prescriptions in Chinese medicine are the collection of herbal medicine prescriptions, the efficiency of these prescriptions were confirmed by the longterm clinical practice，it’s an important treatment method in traditional Chinese medicine. Each of the prescription contains multiple effective components, which be able to regulate multiple targets through molecular network. Especially, it applies secondary effect via neuro-endocrine pathway. Erzhi pill is one of this class of prescription to tonify the liver-kidney in clinic. Our study is based on the analysis of the chemical compounds; preliminary experiments examined estrogen effect and clinic verified effect of Erzhi pill. We proposed that its estrogen effect is the biological foundation of tonifying liver and kidney. The genomic and non genomic outcome of the estrogen effect will examine on cell lines from target organs of Erzhi pill, such as adrenal gland, prostate, breast, bone, brain and liver. In vivo, the effects of Erzhi pill will examine by using mice models include SAMP6 (Osteoporosis model), SAMP8 (Dementia model), Ovariectomized rat and AR, ER, LDLR,ApoE knockout mice, which mimic the “liver-kidney yin deficiency” degenerative disease. Then the biological mechanism of Erzhi pill will be clinical verified by random clinical trials, which performs through recruiting voluntary “liver-kidney yin deficiency” pre-menopause women with hypertension or osteoporosis. Finally, the biological evidence of Erzhi pill will elucidate to enhance its precision in treating chronic disease and provide scientific foundation of treatment of different disease with the same classical prescription in traditional Chinese medicine theory.