关于何首乌致肝损伤的报道引起广泛关注，但关于何首乌致肝损伤的指征成分确证及与肝损伤不同生物学特性的关联性及机制、药物组份之间相互影响等并不清晰。本研究在已有研究的基础上采用关联分析等数据挖掘手段、液质联用技术进一步对何首乌中提取分离部位进行肝损伤-损伤生物学指标-成分及配伍进行分析，明确导致不同肝损伤的生物学特性成分及组份之间配伍影响；同时采用单克隆抗体特异性敲除技术、高内涵筛选等对结果进行验证；采用基因芯片技术、蛋白质及代谢组学等研究致肝损伤的作用机制，同时采用模式生物斑马鱼对相关研究结果进行验证。通过研究可以揭示何首乌中致肝损伤的物质基础与作用机制、主要成分、指征成分及组份配伍影响，为何首乌准确、合理应用，为修订或增加药典何首乌项下及含何首乌中成药用法用量、使用注意等提供理论基础和实验依据，也为类似研究提供参考借鉴。

Numerous studies on Polygonum Multiflorum which could cause liver injury raised widely concern, but the knowledge about the transaction of components, the relevance and mechanism of the active ingredients resulting in liver injury and the diverse biological properties of hepatotoxicity were not distinct. Data mining methods such as association analysis, and the LC/MS were used to further analyze the liver injury, injury characteristics and the compatibility for the extract sites of Polygonum Multiflorum, determining the biological properties, the compatibility between the ingredients and the components of the ingredients which result in diverse liver injury. And the study validated the above results by monoclonal antibodies specific knockout, high content screening, etc. A number of methods such as gene chips technology, proteomics and metabonomics were applied to investigate the mechanism of different types of liver injury caused by diverse extract sites, and then the results were verified by zebrafish as the model organism. The studies are able to reveal what ingredients cause the liver injury, and the compatibility and transaction of the ingredients, the indicated ingredients and components. This research is expected to provide an academic evidence for the reasonable application of Polygonum Multiflorum, supply the amended content under the Polygonum Multiflorum caption of in Pharmacopoeia of The People’s Republic of China with usage and dosage, indicatons, etc.