突变体是经典遗传学和功能基因组学研究中重要的遗传材料。本研究以自然突变的玉米籽粒和叶片双白化突变体为材料，采用图位克隆的方法分离和克隆同时控制玉米籽粒和叶片白化基因。利用电子显微技术观察突变体籽粒和叶片的细胞结构；利用等位性测验、玉米转基因、时空表达模式、蛋白表达、亚细胞定位等方法验证突变基因的功能；开展突变体与野生型的胚乳和叶片的转录组测序，构建突变基因的共表达网络；分析突变基因在不同物种中的进化关系；阐明突变基因在玉米籽粒色素形成和叶片叶绿体建成过程中的作用机制，探讨玉米细胞器质体及叶绿体进化的分子机制。

Mutant is an important material for studies both in classical genetics and functional genomics. In this study, a natural mutant was identified, which affects both seed and leaf color. Positional cloning will be carried out to identify the gene controlling seed and leaf color by using an F2 population derived from a single cross between B73 and the heterozygous mutant. Consequently, the function of candidate gene will be investigated by electronic microscopic observation for the kernel and leave structures, allelism test, transformation-mediated validation in maize, expression profiling, subcellular localization, etc. In addition, the endosperm and leaf of both the wild type and the mutant will be RNA sequenced, and the coexpression regulation network of the target gene will be built. Finally, the phylogenetic analysis of the candidate gene will be performed. All these studies will provide useful information for understanding the molecular mechanism of the candidate gene in regulating the formation of seed color and chloroplast, and the evolution process of chloroplast and kernel chromoplastid in maize.