中文摘要
干旱缺水严重制约着小麦生产的稳定和提高。提高小麦蒸腾效率及抗逆性是促进小麦生产可持续发展的关键。ER基因可调控植物器官的形成和分化,在调控蒸腾效率及抗逆防御反应中发挥着重要作用。因此,项目组克隆了小麦TaER基因,通过表达分析和VIGS分析,发现TaER基因也是调控小麦蒸腾效率的关键基因。为了进一步研究TaER基因的功能,项目组拟利用已获得的过表达TaER基因的12个小麦株系,进一步分析TaER调控小麦蒸腾效率的生物学基础及其调控抗旱耐热性的功能,明确其利用价值;进而通过TaER基因表达差异显著的小麦极端基因型材料,挖掘TaER基因的优异位点,建立鉴定TaER基因优异位点的技术。本研究将促进小麦蒸腾效率和抗旱耐热性的调控机制研究,为小麦抗旱、耐热分子设计育种提供有效工具。
英文摘要
Drought is one of the main limiting factors for wheat growth, development and yield. The increase of wheat production in the limited water condition is of great Increasing the transpiration efficiency and stress resistance of wheat is very important to the stability and improvement of wheat production. ER gene, regulating the formation and differentiation of plant organs, and play an important role on plant transpiration efficiency and stress resistance. So, we cloned the wheat ER gene, and found that TaER is also an key gene on transpiration efficiency in wheat by the expression and VIGS analysis. For further analysis of the function of TaER, the 8 transgenic overexpression lines will be used to understand the biological basis of TaER on wheat transpiration efficiency and its effects on drought or heat resistance. Additionally, the cultivar which have distinctive different TaER expression patterns was used to search the excellent alleles and develop specific markers for check these alleles. This study will promote the analysis of the regulatory mechanism on wheat transpiration efficiency and drought or heat resistance, and providing an effective tool for drought and heat resistance molecular design breeding of wheat.
