溃疡性结肠炎发病率日益上升，被世界卫生组织列为现代难治病之一。固公果根是彝族传统药物，具有涩肠止泻之功。前期我们首次确定固公果根治疗腹泻的有效部位，并发现其能有效治疗DSS诱发的小鼠溃疡性结肠炎。探索性研究发现其通过降低炎症部位TNF-a和IL-6含量，抑制NF-B活性，激活Nrf2-ARE通路，达到抗炎、治疗溃疡性结肠炎作用。因此本项目基于NF-B/Nrf2-ARE通路研究固公果根提取物治疗溃疡性结肠炎的抗炎机制和物质基础。运用多种色谱手段追踪分离固公果根提取物治疗溃疡性结肠炎的物质基础；利用Nrf2 (+/+)ICR和Nrf2 (-/-)ICR小鼠在整体动物水平上评价其对溃疡性结肠炎的防治效果；利用RT-PCR和western-blot在基因和蛋白水平上研究其对炎症介质的mRNA表达和相应调控因子的蛋白表达差异，明确作用机制。研究结果将为该彝族药物治疗溃疡性结肠炎提供理论和试验依据

As the rising incidence of ulcerative colitis (UC), UC is listed as one of the modern cureless diseases by the World Health Organization. Radix Rosae giganteae is the Yi traditional medicine of anti-diarrhea with astringents. The effective part of Radix Rosae giganteae in treatment of diarrhea was firstly determine in our previous research, which showed significantly effect in the treatment of ulcerative colitis in mice induced by DSS. Exploratory study in our group showed that the effective extract of Radix Rosae giganteae can reduce TNF- and IL-6 levels in inflammation part, inhibit NF-kappa-B and active the Nrf2-ARE pathway with anti-inflammatory and treatment of ulcerative colitis. So we will study the anti-inflammatory material basis and mechanism of Radix Rosae giganteae in the treatment of UC based on the NF- kB/Nrf2-ARE pathway. In this project, the functional components to anti-ulcerative colitis were tracked and isolated by a variety of chromatographic separation methods. At the same time, the anti-inflammatory function and mechanism of this extract were evaluated by the Nrf2 (+/+) ICR and Nrf2 (-/-) ICR mice models at the whole animal level of anti-ulcerative colitis. In addition, the expression of inflammatory mediator mRNA and different expressed protein of regulating and controlling factors were determined by using the DNA-ladder and western-blot in the gene and protein level. The results will provide theoretical and experimental basis for this Yi medicine in the treatment of ulcerative colitis.