由番茄黄化曲叶病毒（TYLCV）导致的番茄黄化曲叶病是一种世界范围发生危害的毁灭性病毒病，近年来该病在我国爆发成灾，给当地的番茄生产造成了惨重损失。前期研究发现TYLCV编码的V2与番茄体内谷氧还蛋白（slGRXC6）存在互作，而V2是其侵染寄主所必需的组分，暗示了二者互作可能在TYLCV侵染过程中起重要作用。本项目拟采用激光共聚焦显微技术研究V2与slGRXC6的定位及共定位；通过Y2H、pull-down、BiFC等方法研究二者之间的互作，明确其关键互作功能域/位点，分析其对基因定位及功能的影响；采用QRT-PCR分析TYLCV侵染对slGRXC6的表达调控及其与V2相关性；通过VIGS及转基因手段研究slGRXC6表达受调控后对TYLCV侵染的影响，最终阐明二者互作在TYLCV侵染过程中的作用方式。研究结果对揭示TYLCV侵染过程中的关键因子及阐明TYLCV侵染机理将具有重要意义。

Tomato yellow leaf curl virus (TYLCV), a whitefly-transmitted geminivirus, is one of the most important pathogen of tomato yellow leaf curl disease, which causes devastating losses worldwide, including China in recent years. In previous research, a tomato glutaredoxin (slGRXC6) could interact with V2 protein encoded by TYLCV, which is a suppressor of RNA silencing and essential for virus successful infection. The biological significance of the interaction during the process of TYLCV infection is still unknown. To study the function of these proteins in the virus infection process, we propose to use fluorescence labeling method to clear the protein subcellular distribution and whether they were co-located with each other, use the methods such as Y2H, pull down, BIFC to conform their interaction and identify the motif or sites responsible for the interaction and characterization of the effects of the motif or sites on subcellular localization and function, use QRT-PCR to characterize the expression of slGRXC6 modulated by V2 protein; use VIGS (virus induced gene silencing) and transgene method to clear the correlation of the expression level of modulated slGRXC6 with infection ability of TYLCV. These results are expected to characterize the host factors involved in TYLCV infection, and to promote the understanding of TYLCV infection mechanism.