根系构型形态决定植物生长发育的方向，ABA（脱落酸）在根系构型形成过程中具有重要调控作用；根域限制作为一项突破“根深叶茂”传统栽培理论的果树栽培新技术，可有效提高果实品质和商品果产量。根域限制后葡萄根系ABA大幅升高的同时，根系总量和细根数量极显著增加，表明ABA可能作为根源信号调节了葡萄的根系构型。本研究拟通过根域限制、外源ABA和ABA合成抑制剂处理等来探讨根域限制、根源ABA与葡萄根系构型形态建成的关系；同时结合根域限制解除（容积恢复）、根系密度控制和根域水势实时变化等，分析根源ABA对根域限制诱导的响应及其与根系构型形态之间的关系，了解根源ABA的合成、分配和生理效应；此外采用转录组学技术，分析根系构型形态建成关键基因、ABA生物合成及信号转导通路基因对根域限制和ABA的响应模式，了解根域限制效应的根源信号及其诱导机理，进一步完善根系构型形态建成理论。

Plant growth direction relays on root system architecture, and ABA（absicsic acid） plays an important role in formation of root system architecture. As a new fruit cultivation technology compared to traditional concept, root-zone restriction can effectively repress vegetative growth and improve reproductive growth in grapevine. Former research showed that root sourced ABA level increased remarkably and root system architecture changed significantly under root-zone restriction；did root-sourced ABA act as the signal of root-zone restriction which guide the formation of root system architecture and growth of vine? To clarify this question, grapevine root system will be treated by root-zone restriction, exogenous ABA and FLU(inhibitor of ABA biosynthesis), to analyze the effect of root-zone restriction and ABA on formation of root system architecture. Except the former treatments, recovering restricted root-zone and decreasing root density will also be adapted to investigate the biosynthesis, distribution and physiological effects of root-sourced ABA. Furthermore, highthroughput transcriptome sequencing to reveal root gene expression profiles of grapevines induced by root-zone restriction and ABA. The relationship among root-zone restriction, root-sourced ABA and formation of root system architecture will be discussed. This research is expected to provide theoretical evidence for formation of root system architecture.