水稻细菌性褐条病这一传统次要病害近年来正逐渐上升为我国水稻主要细菌病害之一。为搞清楚该病菌的致病机理，申请者前期已基于基因组和感染转录组成功鉴定了该病菌Ⅰ-Ⅵ型分泌系统和致病岛等编码基因的组成和功能，但对非编码(non-coding, nc) RNAs则缺乏关注。本申请项目拟在生物信息学分析获得353个假定ncRNAs，半定量RT-PCR验证16个感染等条件差异表达ncRNAs，敲除或过表达其中4个影响致病性等细菌学特性的基础上；首先继续Northern杂交验证差异表达ncRNAs；然后过表达和敲除其它12个差异表达ncRNAs，比较各突变体与野生型的细菌学特性，以鉴定致病ncRNAs；最后测定致病ncRNAs突变体的转录组鉴定靶基因，敲除靶基因分析其致病功能及对ncRNAs表达的影响，以明确致病相关ncRNAs的组成及其作用机制，为通过调控ncRNAs表达来控制水稻褐条病提供基础。

In recent years, bacterial brown stripe is gradually becoming one of the main rice diseases in China. In order to understand the pathogenic mechanism of the pathogen, the applicant has successfully identified the composition and function of virulence-related genes such as those encoding pathogenicity islands and the type I-VI secretion systems based on the sequenced genome and transcriptome during in vivo infection of brown stripe pathogen, but little attention was paid to the role of the non-coding (nc) RNAs. Following bioinformatics analysis, a total of 353 putative ncRNAs were predicted, in which 16 ncRNAs confirmed by RT-PCR were found to be differentially expressed during in vivo infection, while there was a difference in the bacteriological characters such as pathogenicity between the constructed 4 knockout or overexpressed mutants and the wild type strain, this proposed project intends to further verify differentially expressed ncRNAs by Northern blot, to identify virulence associated ncRNAs by comparing the bacteriological characters between the wild type strain and the 12 constructed knockout and overexpressed mutants, and to eludicate the regulation mechanism of virulence related ncRNAs by determining the target gene in the term of transcriptomic comparison between the wild type and the ncRNAs mutants, and knockouting the target gene and examing the effect of mutation on the pathogenicity and expression of ncRNA. Results from this study will provide a basis for controlling brown stripe by the modulation of ncRNAs expression.