脑卒中跃居为致死率世界第二和主要致残疾病，脑卒中后疼痛（CPSP）是指卒中后中枢神经系统发生的疼痛。胶质细胞激活是导致CPSP主要原因。我们前期证实电针能降低CPSP大鼠的痛阈值且15Hz效果优于其它频率。此外，电针能下调β-catenin在脑组织表达。我们推测电针通过介导Wnt/β-catenin通路调控星形胶质细胞起到镇痛作用。本研究通过自体血注入模拟临床建立CPSP模型，通过电针“百会”和“足三里”观察CPSP大鼠脑星形胶质细胞变化，检测胶质细胞的生物物理特性高通量表征，观察Wnt/β-catenin信号通路的改变以期阐明电针治疗CPSP的中枢机制，而使用Wnt/β-catenin通路抑制剂Dkk-1能否对电针治疗效果产生影响？本研究拟运用免疫荧光双标技术、微流控分析技术、免疫吸附酶联反应和Western blotting方法为电针治疗CPSP的中枢机制提供依据。

Stroke is a second cause of death and the leading disability in the adult of the world. Central Post-stroke pain (CPSP) is neuropathic pain occurs in the central nervous system after apoplexym, which has ascended to the disease that the mortality rate ranked second in the world and the leading cause of disability in adults. Microglia activation induced central sensitization occurs abnormal pain is the main reason of CPSP. Our previous studies proved that electro acupuncture can reduce the rat models with CPSP’s mechanical pain threshold and heat pain threshold, and can down regulate the expression of β-catenin in brain tissue of rats with CPSP.Thus, we hypothesized that electroacupuncture at "Baihui" and "Zusanli" affecting glial cell differentiation and cell polarity through the Wnt / beta catenin pathway, to maintain the stability of β-catenin in glial cells.This study intends to establish CPSP model to simulate clinical stroke onset by autologous blood injection method, through high frequency electroacupuncture stimulate at "Baihui" and "Zusanli" acupoints in the treatment of CPSP, by observing the changes of intracerebral glial cells after using high frequency electroacupuncture in the treatment of CPSP, to make further detection that glial cell hypertrophy leads to the change of Wnt / β-catenin signaling pathway in order to further elucidate the central regulation mechanismthe of high frequency electro acupuncture for CPSP, and can selective using the inhibitor Dkk1 in Wnt / β-catenin signaling pathway influence the effect of high frequency electro acupuncture for CPSP?This study intends to use the double immunofluorescent labeling techniques, microfluidic analysis technique, enzyme immunoassay and Western blotting method to provide a theoretical basis for the central regulatory mechanism of electro acupuncture in CPSP.