平肝潜阳法作为中医防治高血压的重要治则之一，研究表明能够逆转血管重构，但机制尚未阐明。本项目采用平肝潜阳法经典方剂-天麻钩藤饮为研究工具，以逆转血管重构为切入点，从临床、动物和细胞水平；运用形态学、免疫荧光染色、激光共聚焦和分子生物学方法，结合过表达和抑制表达技术；从3个方面阐明p38MAPK信号通路介导HSP27表达参与平肝潜阳法逆转高血压肝阳上亢证血管重构的作用机制：(1)平肝潜阳法调控MKK3/MKK6-p38MAPK表达参与逆转血管重构的作用机制；(2)平肝潜阳法调节HSP27表达参与逆转血管重构的作用机制；(3)p38MAPK-HSP27信号通路参与高血压肝阳上亢证血管重构的作用机制。揭示平肝潜阳法干预高血压肝阳上亢证是通过调控MKK3/MKK6-p38MAPK-HSP27信号通路的作用机制；有助于阐明平肝潜阳法干预高血压肝阳上亢证的科学内涵，为临床防治高血压提供实验依据。

As one of the important methods to prevent and treat essential hypertension, the study shows that the method of calming liver and suppressing Yang can reverse the vascular remodeling, but the mechanism has not been clarified. This project uses the classical prescription of calming liver and suppressing Yang method-Tianma Gouteng decoction as a research tool, to reverse the vascular remodeling as a starting point, from the level of clinical, animal and cell, by morphology, immunofluorescence staining, confocal laser and other molecular biological methods, combined with the over expression and down expression technology. There are three aspects to clarify the mechanism in calming liver and suppressing Yang calming method intervene vascular remodeling in essential hypertension with hyperactivity of liver-Yang syndrome through the p38MAPK signal pathway regulating the expression of HSP27: (1) The method of calming liver and suppressing Yang calming could reverse vascular remodeling by involving in regulating the expression of MKK3/MKK6-p38MAPK mechanism. (2) The method of calming liver and suppressing Yang calming could reverse vascular remodeling by involving in regulating HSP27 expression mechanism. (3) The p38MAPK-HSP27 signaling pathway is involved in vascular remodeling mechanism on essential hypertension with hyperactivity of liver-Yang syndrome. The method of calming liver and suppressing Yang treatment on essential hypertension with hyperactivity of liver-Yang syndrome is illustrated through the mechanism of MKK3/MKK6-p38MAPK-HSP27 signaling pathway. These results can help to clarify the scientific connotation of calming liver and suppressing Yang method on treatment of essential hypertension with hyperactivity of liver-Yang syndrome. The findings suggest that it can provide some experimental basis for clinical prevention and treatment of essential hypertension.