中文摘要
油楠也称“香脂树”,隶属于苏木科油楠属,因其树干可分泌树脂油,故在开发药物制剂和天然精油等方面具有重要用途。目前,野生植株泌油机制尚未探明和泌油性状准确鉴定困难等问题,限制了油楠遗传改良和种质资源创新利用。为此,本项目基于前期初步筛选的高、低泌油野生油楠单株和营建的子代测定林材料,通过采集树皮、叶、枝干和树干木芯试样,分析野生植株树皮、叶和种实的外部形态变异,解析不同器官的分泌道结构特征及其之间的关联性,建立树干分泌道结构特征与泌油性状的回归关系;同时,采用SSR分子标记,基于遗传变异评价,开展分子标记与泌油性状关联的位点分析和特异性SSR标记的筛选;最后综合植株外部形态、内部结构和SSR标记,筛选出与泌油性状显著相关的特异性表型或分子标记,并利用树干分泌道与泌油特性的回归方程,评价油楠子代的泌油性状,验证特异性标记的可靠性和一致性,从而为探明油楠泌油机制和快速鉴定特异的种质资源提供参考。
英文摘要
Sindora glabra Merr. ex de Wit, belonging to the Caesalpiniaceae family, is endemic to the province of Hainan,China, and is called as “balsam tree” due to the high content of oleoresin in the tree trunk, serving a great potential utilization in developing inter and external medicine and professional care products. Up to date, some progresses have been made in the production of oleoresin for S. glabra; however, the mechanism on oleoresin exudation is not well understood and the methods to identify the exudating property are still lacking, which restrict genetic improvement and utilization of germplasm resources of S. glabra. .With a better understanding of the characteristics of oleoresin producing and the mechanism as well as assessment of oleoresin exudation for this species, this project will carry out studies on identification of oleoresin exudation traits for wild germplasms of Sindora glabra based on both phenotypic and SSR molecular markers and on verification of the reliability of them utilized in the evaluation of target characters of the progeny of S. glabra. Based on the selection of each five plants with high and low oleoresin exudation characters and the establishment of progeny test plantation, external morphological variation will be analyzed, and characteristics of analytic secretory canal structure from different organs of plants and relationship between them will be evaluated, and the regression relationship between the characteristic of secretory canals and oleoresin secreting traits will be fitted after the collection of barks, leaves, branch wood and the trunk wood core samples. Meanwhile, the genetic variation and locus related to oleoresin will be analyzed by using SSR molecular markers, aiming for screening the specific SSR markers. Finally, in combination with plant external morphology, internal structure and SSR marker, specific phenotype or molecular markers significantly associated with oleoresin production will be selected and utilized in verifying reliability and consistency of specific markers in assessing the oleoresin production characters for progeny individuals. Through the implementation of this study, the variation of both external morphology and interanl structural features of secretory canals would be revealed, and the interrelationship between genetic markers and oleoresin production characteristics would be identified, in an attempt to offer scientific references to reveal the mechanism of oleoresin exduation, to identify the excellent individual of high yield of oleoresin and to strengthen directional breeding.
