整合景观要素探讨物种的微进化过程受到普遍关注。本项目以连续分布于内蒙古高原、鄂尔多斯高原与黄土高原（部分）的短花针茅草原为研究区域，依据规则（网格）取样的方法设置样地，采集短花针茅个体样本；利用SSR分子标记手段，获取短花针茅种群遗传信息；利用传统群体遗传学与景观遗传学等方法，分析短花针茅种群遗传多样性、遗传结构、基因流以及遗传不连续体等特征；最后，集成气候、地形地貌、土地利用等多尺度空间专题数据，利用GIS技术与地统计学等方法，探究影响短花针茅群体遗传分化的关键环境要素。通过上述较为系统的研究，阐明自然与人为因子对短花针茅遗传多样性与遗传结构的影响，明晰不同环境要素的尺度效应，从而揭示出短花针茅种群遗传分化的机制。其结果不仅丰富了景观遗传学内容，而且有助于深刻认识短花针茅草原（乃至欧亚草原东部）的演化，对于未来气候变化和人类活动共同作用下针茅草原的生物多样性保护也具有重要的参考价值。

Integrating landscape factors to address species microevolution processes has being received more attention. This study was carried out in the region of Stipa breviflora grassland, which is distributed continuously along Inner Mongolia plateau, Ordos plateau and parts of Loess plateau. Field sites were selected according to the grid method, and individual samples of S. breviflora were collected in each site. Genetic information was acquired using SSR markers. Characteristics of genetic diversity, genetic structure, gene flow and genetic discontinuities of the population were analyzed by applying both traditional population genetics approaches and landscape genetics approaches. Finally, through incorporating spatial thematic dataset on different scales such as climate, topography and land use with genetic information by means of GIS and geostatistics tools, key environmental factors affecting genetic differentiation of the species were illustrated. This study could explore genetic characteristics of S. breviflora population systematically, and show impacts of natural and anthropogenic factors derived from different scales on genetic diversity and structure of the population. It was also helpful to clarify the scale effect, and then to demonstrate the mechanism of genetic differentiation of S. breviflora. The results were expected to enrich the study of landscape genetics, to understand the evolution of S. breviflora grassland and/or the east part of Eurasian steppe. The study also provided information for biodiversity conservation of Stipa grassland affected by both climate change and human activities in future.