中文摘要
近年来我国水稻产区干旱、盐碱或冷害频发,常造成水稻大面积减产,严重威胁国家粮食安全。为此,分离鉴定调控水稻应答多种非生物逆境胁迫关键基因的功能并阐明其调控机理,采用分子育种技术改良水稻的综合抗逆性势在必行。本课题在前期研究中分离的编码1R类别MYB转录因子基因OsMYBR1的表达受多逆境诱导,该基因定位在细胞核,具有转录激活活性,并且超量表达可提高转基因水稻对干旱、高盐和冷胁迫的抗性。在此基础上,本项目拟进一步对该基因的功能及其调控机理进行研究。预期可阐明OsMYBR1基因提高水稻多种非生物逆境胁迫抗性的生物学功能及其调控机理,为解析水稻体内多种非生物逆境胁迫信号传导途径交叉的调控网络和分离对多种非生物胁迫具有耐受性的关键因子提供理论依据,并为改良水稻的综合抗逆性提供基因资源。
英文摘要
Drought, salt and cold stresses caused a great yield loss in Chinese main rice.producing areas in recent years, and will threaten the food security. Thus, it is extremely urgent to research the function and clarify the regualtion mechanism of the factor controlling the responsiveness of rice plant to multiple abiotic stresses, and develop new multiple abiotic stress tolerant rice varieties by molecular breeding. Rice gene OsMYBR1was one of the genes identified to be induced by multiple stresses, encoding a nucleus-localized protein of R1-MYB, has transcriptional activation activity. Preliminary results showed that overexpression of OsMYBR1 in rice enhanced the drought, salt and cold tolerance in transgenic plants. The program will be carried out to further study the function and regulation mechanism of OsMYBR1. The expected results will elucidate the biological function and regulation mechanism of OsMYBR1 involved in drought, salt and cold tolerance. This research will provide theoretical reference for analyzing the regulatory networks and cross-talk among different signal transduction pathways, and isolating the key factors with tolerance to multiple abiotic stresses, and provide a candidate gene to develop new rice varieties with comprehensive tolerance ability.
