中文摘要
藤壶分布于世界各地,是主要的污损生物之一。金星幼虫是藤壶幼虫时期的最后阶段,担负着附着变态的过程。附着点一旦确定,金星幼虫分泌胶黏剂将其自身永久性的固着在附着点。藤壶胶蛋白是藤壶胶中的主要成分,说明胶蛋白在藤壶的固着过程中起着重要的作用。显然,藤壶胶的黏附作用远强于金星幼虫的胶黏剂。分析胶蛋白成分的异同是了解金星幼虫胶黏剂与藤壶胶作用强弱的必要前提 。但目前国内外尚未系统分析金星幼虫胶黏剂及藤壶胶中胶蛋白成分的异同。本项目以红巨藤壶为研究对象,通过转录组,蛋白质组的研究,比较金星幼虫黏胶剂及藤壶胶中胶蛋白的成分,并分析新型胶蛋白的物理化学特性,进一步了解金星幼虫附着和成体藤壶固着的机制,并分析其异同。
英文摘要
Barnacles are one of the major fouling organisms, distributed around the world. Cyprid is the last larval stage, responsible for exploring the substratum surface, and settlement (a process including attachment and metamorphosis). Once the site decided, cyprid secretes permanent adhesive to settle. Cement proteins are the main component in the secreted cement from adult barnacles, suggesting cement proteins play a major role in the process of barnacle settlement. Obviously, the barnacle cement is far stronger than that from cyprid. But the mechanism is still unclear. Comparison and analysis of the protein components of both cyprid and adult cement are required to understand the mechanism underlying the phenomena. Megabalanus Rosa will be used in this study. The similarity and difference will be systematically analyzed between the cyprid and the adult cement by transcriptomic and proteomic approaches. The novel cement proteins will be also characterized in this study.
