申请人石磊长期研究细胞内囊泡运输，在囊泡膜融合方向取得重要成绩：（1）构建体外融合实验系统，在单分子水平上成功解答单次囊泡融合需要几个SNARE蛋白，解决了该问题长期以来的激烈争论，研究结果一经在Science发表便被美国《科学家》杂志专题报道，评论为“进一步突显了SNARE蛋白在囊泡融合中的重要性”，被同行认为是该领域的“重要进展”；（2）揭示囊泡融合调节蛋白Munc18新的作用机制；（3）揭示真核细胞中cortical ER的形成机制。已发表SCI文章10篇，其中以第一和通讯作者在Science，Nat. Protocols，Mol. Biol. Cell, BMC Biology等杂志发表文章6篇，文章他引超过160次。获2015“求是杰出青年学者奖”。病毒侵染细胞涉及囊泡运输与膜融合，基于上述工作，计划针对HIV侵染细胞的膜融合过程开展研究，解析病毒融合蛋白GP41的作用机制。

Lei Shi’s major studies focus on intracellular vesicles transport. Lei Shi’s work is to unravel the mechanism of vesicle fusion driven by SNARE proteins. The major achievements include: (1) answer the question “How many SNAREs are needed in a vesicle fusion event?” at single molecule level by reconstitution of a novel fusion system, settle down the long-term debate on this issue. Soon after the publication on the “Science” journal, this work is chosen and reported by “The Scientist” and commented as “This further emphasizes the importance of these proteins in the process of membrane fusion”. This work is also recommended by F1000 and regarded as “significant advance”; (2) unravel the new mechanism of regulatory protein Munc18; (3) reveal the mechanism of cortical ER formation in eukaryotic cells. Lei Shi has published 10 research papers that had been cited by others for more than 160 times. Lei Shi is first author or corresponding author of 6 papers published on Science，Nature Protocols，Mol. Biol. Cell, BMC Biology and so on. Lei Shi achieved “Qiushi Young Scholar Award” in 2015. Based on above work, Lei Shi plans to study the mechanism of HIV virus fusion mediated by GP41 virus fusion protein.