神经源性膀胱是脊髓损伤(SCI)后严重的合并症，是截瘫患者晚期的主要病死原因。SCI患者80%以上为骶上脊髓损伤，其所导致的膀胱功能障碍主要表现为逼尿肌反射亢进，膀胱储尿困难、尿失禁。抑制脊髓损伤后继发神经元凋亡，促进膀胱逼尿肌舒张可有效改善骶上脊髓损伤后神经源性膀胱的功能，PACAP-cAMP信号通路在抑制神经元凋亡及促进膀胱逼尿肌舒张方面均有重要作用。本项目以此为切入点，以骶上脊髓损伤所致的尿失禁大鼠模型为受试对象，采用电针治疗，运用尿流动力学、光镜、免疫组化、RT-PCR、Western-blot等多种检测手段，观察膀胱尿流动力学及逼尿肌组织形态，脊髓损伤局部组织形态及凋亡相关蛋白含量，胞浆内PACAP、cAMP含量，PACAP-cAMP信号通路下游环节中各调节蛋白磷酸化状态及相关基因表达量等的变化，探讨电针治疗骶上脊髓损伤后神经源性膀胱可能的效应机制。

Neurogenic bladder is a severe complication of spinal cord injury (SCI), the main cause of death in late patients with paraplegia. More than 80% of SCI patients are injured above the sacral segment, which lead to bladder dysfunction mainly as detrusor hyperreflexia，urinary bladder storage problems, and urinary incontinence.Inhibiting secondary synaptic and neuronal apoptosis after spinal cord injury, and promoting the bladder detrusor relaxation can effectively improve the neurogenic bladder after spinal cord injury above sacral segment. PACAP-cAMP signal pathway plays an important role in inhibiting synaptic and neuronal apoptosis and promoting the bladder detrusor relaxation.The project takes PACAP-cAMP signal pathway as a starting point, rat model with urinary incontinence caused by spinal cord injury above sacral segment as subjects,uses electroacupuncture as treatment, uses the urine flow dynamics, light microscopy, immunohistochemistry, RT-PCR, Western-blot and other means of detection, by observing the changes of urodynamics and detrusor tissue morphology, the changes of local tissue morphology and apoptosis related protein of spinal cord injury，the changes of PACAP, cAMP content in the cytoplasm, the changes of phosphorylation of regulatary protein and gene expression in the downstream of the PACAP-cAMP signaling pathways , to explore the possible effect mechanism of electroacupuncture on neurogenic bladder after spinal cord injury above sacral segment.