如何促进应激颗粒(Stress Granule, SG)是中风修复的关键问题, RNA结合蛋白为调控SG关键分子。在中医开窍理论指导下，我们发现，开窍急救名方醒脑静及其配伍开窍药促进SG形成和RNA结合模序蛋白3（RBM3）表达上调。因此，提出“开窍有效成分选择性作用于RBM3所产生的SG，抗脑缺血损伤的整合作用"假说。以无血清诱导细胞应激颗粒为模型，构建RBM3启动子驱动的荧光素酶报告基因系统，阐明RBM3对开窍有效成分反应元件，与RBM3启动子作用的靶蛋白。建立RBM3调控 SG靶mRNA的关键技术，采用RNAi技术封闭RBM3，或转染RBM3到 SG细胞模型中，阐明开窍有效成分调控RBM3影响 SG形成，抗细胞凋亡损伤分子机理。在脑缺血动物模型进一步阐明开窍影响RBM3和SG，抗脑缺血凋亡机制。本项目促进中医开窍理论发展，为开窍在临床防治中风提供理论依据，为新一代抗中风新药奠定基础。

The key of neuronal repair is how to activate Stress Granule (SG) in stroke, and the RNA-binding protein just is the important molecule in regulating SG. Under the theory of resuscitation in traditional Chinese medicine, XING NAOJING, as the famous prescription in emergency resuscitation，was found to induce the SG and up-regulate the RNA-binding motif protein 3 (RBM3). So, we provided a hypothesis that the effective ingredients of resuscitation selectively act on RBM3, which induce the Stress Granule, have the integrated function to resist the neuron damage in cerebral ischemia. Taken the cell induced with free-serum medium as the SG cell model, we construct the RBM3 promoter luciferase reporter gene system to illuminate the effective cluster response element of resuscitation medicines and target binding proteins with RBM3 gene. And we build the regulating technique of RNA-binding protein targeted to SG mRNA, and use RNAi methods to block RBM3 gene express, or transfect RBM3 gene into the SG cell model to illustrate the resistant apoptosis mechanism of RNA-binding proteins regulated by resuscitation effective ingredient acting on Stress Granule. In the cerebral ischemic animal model, we further to confirmed the integrate mechanism of effective ingredient targeting RNA-binding protein and Stress Granule to resist against the apoptosis. Our research will promote the resuscitation theory development, and provide the foundation for resuscitation theory and an innovation in drugs on treatment of stroke.