外泌体（exosome）是细胞分泌的囊性小体，具有抗原提呈、细胞间物质转运和信息传递等特性。本课题组前期研究发现心肌细胞分泌的exosome对甲基苯丙胺依赖有干预作用，经过钩藤碱处理心肌细胞后，exosome的抗依赖作用更显著。推测心源性exosome通过向中枢递送重要信号分子和遗传物质而影响CNS。对exosome特征和功能的研究，可揭示一个由exosome介导的遗传信息传递方式和表达调控网络，从一个全新的角度注释和阐明药物成瘾机制，从中寻找中药抗药物依赖的新途径。本项目通过甲基苯丙胺依赖细胞模型与动物模型，借助于分子生物学方法与基因芯片技术，研究心肌细胞源性exosome的生物特性及其对甲基苯丙胺依赖的影响。阐明exosome对CNS的作用机制，同时明确钩藤碱对exosome的诱导作用和调控机制。本项目创新性地把exosome引入毒瘾医学和中药学领域中，开拓了中药-外泌体应用的新途径。

Exosomes are nanosized vesicles secreted by cells, which possess various biological characters such as antigen presentation, cell-to-cell material transport and information transmission. The previous studies in our research group found that the exosome secreted by myocardial cells exerted inhibitory effect on methamphetamine dependence, and the exosome exposed to rhynchophylline could enhance significantly the inhibitory effect. Therefore, we speculate that cardiogenic exosome can regulate CNS by carrying important signaling molecules and genetic materials to CNS. The study on exosome features and functions will reveal an exosome-mediated genetic information transfer and expression regulation network, from different from traditional notes, elucidate the mechanism of drug addiction, and look for a new pathway of traditional Chinese Medicine anti addiction. this project will study the biological characters of cardiogenic exosome, its material basis and its effects on drug addiction via the cell and animal models of methamphetamine dependence and the aid of molecular biology methods and gene chip technology. In addition, this project will also study the induction of rhynchophylline on exosome and its regulatory mechanism. The project introduces innovatively exosome to the fields of drug addiction and traditional Chinese medicine, open up a new pathway of Chinese Medicine - exosome application.