耐寒植物珠芽蓼（Polygonum viviparum）是高寒草甸的优势种和建群种，分布范围广，营养丰富，具有较高的饲用价值和药用价值。但植株体内多酚类化合物含量较高，影响了家畜对营养物质的吸收和利用的有效性。漆酶可以有效降解植物多酚类化合物。本项目拟筛选高效且耐逆性强的产漆酶真菌，构建低成本且高效降解多酚类化合物的混合菌群和复合酶系，结合相关物理和化学技术，探讨利用微生物技术和物理、化学手段破解珠芽蓼植株体内多酚类化合物的新途径。并阐明以下几个科学问题：1）植物多酚类化合物是如何抗生物降解的？2）产漆酶微生物是如何降解植物多酚类化合物的？3）降解多酚类化合物和提高植物细胞壁物质转化效率的可能途径有哪些？研究结果不仅可以丰富青藏高原高寒草地耐低温产漆酶真菌资源的认识，而且为提高珠芽蓼多酚类化合物的生物利用度和活性提供理论依据，同时也有助于为将来开展高寒草地耐低温微生物多样性的保育。

Polygonum viviparum,a kind of cold-resistanct plant,is dominant and constructive species of alpine meadows that has wide distribution in Qinghai-Tibet plateau,which not only forage value due to the rich nutrient, but also high medicinal value.However,The plant polyphenolic compounds content is higher to affect livestock to absorb and utilize the nutrients.Laccase can effectively degrade or removal of plant polyphenolic compounds.So,This study was designed to screen and look for cold-adapted producing-laccase fungi strains,which has strong resistance against enviornment conditions especially temperature,meanwhile to build combinations strains and multi-enzymes fungi system that could degrade the plant polyphenolic compounds,combined with the related physical and chemical technology to explore to break the polyphenolic compounds by new means of biotechnology,physical and chemical.And to clarify the following scientific questions: (1) How plant polyphenolic compounds are resistant to biodegradable? (2) How do producing-laccase fungi degrade the polyphenolic compounds？ (3) Which are possible ways for degradation of polyphenolic compounds and to improve the conversion efficiency of the plant cell wall matter? The results not only can enrich cold-adapted producing-laccase fungi of alpine meadow on the Qinghai-Tibet plateau,but also provide a theoretical basis for improving the bioavailability and activity of Polygonum viviparum,and help to carry out biodiversity conservation on cold-adapted microorganisms in the future.