广泛性焦虑症(Generalized Anxiety Disorder，GAD)是临床常见病以及多发病。腹部推拿作为其治疗手段之一，具有良好的临床疗效。本研究基于前期研究基础，以腹部推拿核心手法"摩腹法"为干预手段，提出"摩腹法作用于腹部，影响情感环路，通过对情感环路多个核团整体调节，达到治疗GAD作用"的理论假说，拟以慢性情绪应激法建立模型，检测GAD大鼠肠组织及情感环路中GAS、NPY、CGRP神经肽含量变化、应用fMRI技术探索摩腹干预情感环路的敏感区、运用HRP示踪技术观察情感环路各核团HRP标记细胞表达、采用HE、MRS技术检测摩腹法干预前后情绪环路形态、功能以及各核团结构变化，综合以上研究结果加以对比分析。揭示情绪环路在GAD调节中的作用，以及摩腹法调节情绪环路干预GAD的中枢机制。该项目将进一步深化腹部推拿基础研究，对促进推拿学科发展具有重要意义，为腹部推拿临床推广提供依据。

Generalized anxiety disorder(GAD) is a kind of neurosis with anxiety-based ,which is Clinical common and frequently-occurring disease. Abdominal massage is a method to cure this disease ,which has a good clinical effect.Basing on the earlier period research foundation, taking "Rubbing abdomen" which is as the intervention method as the intervention method ,the study team proposes the hypothesis: when "Rubbing abdomen" acting on the abdomen ,by means of brain-gut axis neural pathways, it could affect mood ring role.The study will detect the changes in the intestinal tissue and emotional loop GAS, NPY, CGRP levels of neuropeptide of rats model of GAD,and observe sensitive areas of rat GAD emotional loop morphology,using HRP tracer techniqueto observe the the neurons of Emotional loop,function and changes in the organizational structure of each nucleus before and after intervention,and based on the above analysis of the results to be compared. Determining the role of a bridge of neural pathways of brain-gut axis,and Observing the action of "Rubbing abdomen" on Emotional loop , so the central regulatory mechanisms would be figured out.After arrange and analyse indexes, this project has the important practical significance for abdominal-Tuina in the treatment of functional gastrointestinal disease theory and clinical practice and provide the scientific basis for the system of abdominal-Tuina mechanism in this project.