“方证相关”蕴涵着中医辨证论治规律，既往多强调方剂对机体生物效应的作用，忽视证候的机体反应状态对方剂的适应性，其关键在于切中病机。本项目结合前期工作，以逍遥散-肝郁脾虚证方证，针对肝郁与脾虚的证候病机，提出“逍遥散-肝郁脾虚证方证相关的生物学基础可能与‘情绪-食欲调节网络’的差异基因和DNA甲基化有关”的假说，开展（1）UPLS-MS/MS联用方法，建立逍遥散指纹图谱（2）研究肝郁脾虚不同证候病机状态下，体液及靶组织中该方证相关的药效成分并进行网络分析（3）转录组学、表观遗传学方法，研究该方证与肝郁脾虚病机密切相关的“情绪-食欲调节网络”差异基因和DNA甲基化差异基因（4）以中药小分子单克隆抗体技术，在细胞水平上求证该方证相关的物质基础（5）临床以抑郁症肝郁脾虚证患者为对象，进一步探索该方证相关生物学基础。这对于深化经典方剂方证相关的理论认识和辨证论治个体化诊疗思想具有重要意义。

Correlation between formula Xiaoyaosan and syndrome contains TCM discipline of syndrome differentiation and treatment. In the past, researchers always focused on the biological effects of Chineses formula on the body, but ignored the adaptability of state of body's reaction to the formula itself. The key is to focus on the pathogenesis. Based on the previous works, in this project, selected the correlation between Xiaoyaosan and syndrome of stagnation of liver qi and deficiency in the spleen as example, based on the pathogenesis of syndrome of stagnation of liver qi and syndrome of deficiency in the spleen, we put forward the hypothesis that: biological basis of correlation between xiaoyaoan and syndrome of stagnation of liver qi and deficiency in the spleen might be connected with differential gene of emotions - appetite regulating network and differential gene of DNA methylation. The project contents were as follows: (1) fingerprint of Xiaoyaosan was established with UPLS- MS/MS method; (2) under different state of syndrome pathogenesis of syndrome of stagnation of liver qi and deficiency in the spleen, study the effective component of the correlation between the formula and the syndrome in the body fluid and target tissues; (3) study the differential gene of emotions - appetite regulating network and differential gene in DNA methylation by the methods of transcriptomics, epigenetics, and bioinformatics; (4) at a cellular level, identify the material basis of the correlation between the formula and the syndrome using the TCM small molecule monoclonal antibody technology; (5) take depression patients with the syndrome of stagnation of liver qi and deficiency in the spleen as the objects, further explore the biological foundation of the correlation of Xiaoyaosan and syndrome of stagnation of liver qi and deficiency in the spleen. This research will be of great importance in deepened understanding the theoretical knowledge of correlation of formula and syndrome of classic formulae, and will be important of understanding the individualized diagnosis and treatment ideas based on TCM syndrome differentiation and treatment.