中文摘要
茄子单性结实性能克服低温引起的落花落果障碍,坐果能力增强,产量显著提高,同时还可改进果实品质,降低栽培成本。茄子单性结实相关基因的功能鉴定和调控研究对阐明单性结实机制,指导单性结实品种的育种工作具有十分重要的意义。. 本试验以茄子单性结实相关基因SmIAA19、SmAGPP和SmMsrA为研究对象,利用GFP标记方法,对其进行亚细胞定位,以期确定该基因表达蛋白质在细胞中的分布;构建克隆基因的VIGS表达载体、RNAi和过表达表达载体,通过农杆菌介导的遗传转化方法获得茄子沉默植株和转基因植株;分析转化植株在其生长发育过程中的生物学特性、单性结实性及转基因的表达,明确茄子单性结实相关基因SmIAA19、SmAGPP和SmMsrA的功能及可能的作用机制,为阐明茄子及园艺植物单性结实形成的分子机制奠定理论基础。
英文摘要
Parthenocarpy can overcome the problem of flower and fruit drop due to low temperature, increase fruit setting, raise yield significantly, and can also improve fruit quality and reduce the cultivation cost in eggplant.To identify fuction of the parthenocarpy related genes and study their regulated patterns are of great significance in clarifying the parthenocarpic molecular mechanism and guiding parthenocarpic breeding.. In this study in order to identify the fuction of parthnocarpy related genes, SmIAA19、SmAGPP and SmMsrA, green fluorescent protein (GFP) technology was used to detecting their expression proteins subcellular location. Their overexpression and RNAi expression vectors were constructed and transferred into eggplant by agrobacteria mediated genetic transformation method. During the transformed plants growing and developing, we investigated their biological characteristics, parthenocarpic ability and expression of transgenic gene, and analysed the function and possible mechanism of SmIAA19、SmAGPP and SmMsrA gene. These researches established the theoretical foundation for clarifying the parthenocarpic molecular mechanism of eggplant and horticultural crops.
