炭疽病(Colletotrichum spp.)是一种在世界范围内严重危害辣椒生产的病害。本课题组最近的研究表明，重要的抗源材料“PBC932”在绿果期和红果期的抗性是显性的，其主效的抗性基因都位于P5染色体的相近位置，重组单株的出现表明控制绿果和红果抗性的基因遗传位点不同。本项目，拟以“PBC932”来源的BC3S1群体为试材，利用KASPar 和QTL-seq技术进行P5染色体上抗尖孢炭疽病主效基因的精细定位，然后结合基因组信息分别克隆控制绿果期和红果期的主效基因，再对候选基因通过表达分析、病毒诱导基因沉默、RNAi和过表达遗传转化等进行功能验证和比较，并对不同果实发育时期的抗性差异进行机理分析。

Anthracnose (Colletotrichum spp.) is a serious disease worldwide in pepper (Capsicum spp.) production. Based on our recent research, inheritance of resistance to C. acutatum from a Capsicum chinense accession (PBC932) detached mature green and mature red fruit is dominate. Main effect QTLs located in a close marker interval on P5 chromosome for both fruit stages and resistant criteria were revealed. Identification of recombinant individuals suggested that resistance in green versus red fruit may be controlled by distinct genes within the QTL interval on P5.In this project, we plan to use BC3S1 population from “PBC932” as material. KASPar and QTL-seq will be employed to make a fine mapping of the major QTL resistant to Colletotrichum acutatum on P5 with the genome sequences as the reference. Candidate resistant genes detached mature green and mature red fruit are going to be cloned based on fine mapping and bioinformatics analysis. Gene function will be proved and analyzed by expression, VIGS, and RNAi technology to set up a scientific model of the mechanism.