本项目依据“治未病”思想，拟证实针灸预处理对卵巢储备功能的保护效应，并提出保护机制的新假说，即：雄激素受体（AR）介导的雄激素稳态平衡是针灸预处理保护卵巢储备功能的关键因素，并通过对卵泡刺激素受体（FSHR）和miRNA-125b的调节，提高FSH敏感性，避免卵泡闭锁，从而促进卵泡正常发育。因此课题组将运用免疫组化、Elisa和RT-PCR等技术，通过观察造模前针灸和造模开始同时针灸对卵巢储备功能减退模型大鼠卵巢储备能力、血清雄激素水平、AR相关信号通路指标的影响，明确针灸预处理对卵巢储备功能的保护效应，及“未病先防”“既病防变”的效应差异，并进一步分析AR相关信号通路调控雄激素稳态平衡在针灸预处理保护卵巢储备功能中的关键作用，为卵巢储备功能减退（DOR）和卵巢早衰（POF）的针灸防治提供实验依据。

Based on preventive treatment, this project would like to confirm the protection mechanism of acupuncture and moxibustion pretreatment's function of ovarian reserve, and propose a new hypothesis: the androgen balance mediated by AR is a key factor of acupuncture and moxibustion pretreatment's function of ovarian reserve, which could promote the follicle growth by increasing FSHR levels and prevent of follicular atresia by inducing the expression of miR-125b. Therefore, the research group will use immunohistochemistry,Elisa and RT-PCR to observe the index of DOR rats before and after acupuncture and moxibustion pretreatment, including ovarian reserve function (follicle number, AMH, etc.), the level of serum testosterone(T, DHT and DHEA),FSHR protein and AR mRNA expression of ovarian tissue, miR-125b expression of granulosa cells, so as to observe the protective effect and effect difference of ovarian Reserve, further analyze the relationship between androgen balance and acupuncture effect through AR signaling pathway, whether miR-125b and FSHR are the key fators. This project provides a new experimental evidence for preventive treatment of DOR and POF with acupuncture and moxibustion.