孕妇直接或环境性吸烟率极高，能诱发新生儿长期肺发育不良，对此治疗是亟待解决的世界性难题。最新研究发现PPARγ和Wnt/β-catenin信号通路等是孕期尼古丁暴露新生儿肺发育不良的关键途径。电针治疗是祖国医学瑰宝，具有明显的神经-内分泌调节及肺脏的保护效应。预实验申请者和美国Prof. Rehan VK联合研究，发现电针可明显促进子代大鼠肺PPAR-γ合成，阻抑Wnt/β-catenin信号通路活化等。本项目拟以孕期和哺乳期尼古丁暴露子代大鼠肺部PPARγ和Wnt/β-catenin信号通路等为切入点，应用HE染色、western-blot、real-time PCR、免疫荧光染色和激光共聚焦显微成像等技术，明确不同经穴效应程度和分布情况，证实电针不同效应程度的经穴可能通过调节大鼠神经-内分泌发挥对新生鼠肺发育的保护效应，且机制存在差异。为电针防治新生儿肺发育不良提供理论依据和新的靶点。

There is a high ratio of direct and passive smoking in pregnant women, which may induce long-term pulmonary hypoplasia in the newborns. It is a serious problem to be solved urgently worldwide. New studies have revealed that the PPARγ and the signal paths of Wnt/β-catenin are the critical paths when the newborns are exposed to nicotine in the pregnant period. Electro-acupunctue therapy is the treasure of Chinese medicine, which is able to regulate nerve-endocrine and protect the lung. The applicant and American Prof. Rehan VK studied jointly in the early preliminary experiment, who have found that electro-acupuncture applied to ST 36 in rats exposed to nicotine in the pregnant and lactation periods could markedly promote PPAR-γ synthesis of the newborn rats' lung, inhibit activation of Wnt/β-catenin signaling pathway, et al. Our research program tries to use the PPARγ and Wnt/β-catenin signal paths, etc. as an entry point to clarify the effect and its distribution of various acupoints, and to confirm that it can regulate nerve-endocrine of rats to protect lung development of offspring, as well as varied mechanisms by applying HE staining, western-blot, real-time PCR, immunofluorescent staining, and laser confocal microscopic imagining. It is expected that the study will provide theoretical foundation and new target spots for protection and treatment of pulmonay hypoplasia of the newborns intervened by electro-acupuncture.