近年来，在中俄地区多次爆发手足口病、EV71感染以及HIV-TB共感染疫情，复杂型别人类病原菌感染性疾病的大规模爆发或流行趋势严峻，已经成为国际上严重的公共卫生问题。型别复杂的病原菌可以引起的疾病类型较为复杂，而且不同型别的病原菌可以引起相同的症状，同一型别的病原菌同时也可以引发不同的临床表现，这为复杂型别病原菌的临床诊断与防治带来了巨大的挑战。病原菌快速准确的分型鉴定是复杂型别病原菌针对性防治过程中最为关键的一环，亟待建立一种基于全基因组信息挖掘及病原菌-宿主相互调控分子分型的新模式。本项目将通过针对于分子分型方法的学术交流，对病原菌分子分型新方法进行深入细致的交流探讨；整合双方在基因组条形码可视化及条形码欧式矩阵分析技术和分子组学分析技术上的优势，探求一种更为优化的、普适的病原菌分子分型技术。这将是以往单一生命科学研究及计算科学研究都无法完成的全新病原菌分型技术，必将提高我国在病原菌分子分型领域的国际影响力。

In recent years, it is commonly reported that hand-foot-and-mouth disease, enterovirus 71 infection and HIV-TB co-infection have outbroke several times in China-Russia region. The large-scale outbreak tendency of human pathogen infectious diseases, that caused by bacterium with complex types, has became a serious public health problem in the world. Pathogens with complex types would induce many kinds of diseases. While different types of pathogens can cause the same symptoms, the same types of pathogens can also cause various symptoms, which phenomenon has brought us great challenges to the clinical diagnosis and control of the diseases caused by pathogens with complex types. For fast and exact identification of pathogens is the most important step in the control of human pathogen infectious diseases, it is urgent to build a new molecular subtyping method that based on the whole genome information mining and pathogen-host mutual regulative molecular subtyping. This project will build intensive communications on the topic of novel pathogen molecular subtyping methods. Through integrate the advantages in genomic bar-coding visualization, European matrix analysis and molecular omics analysis technology, we want to explore a more optimized, universal pathogen molecular classification method. It will be an amazing technology that the past biological and computational researches cannot realize, and this technology will promote the international influence of China in the field of pathogen molecular subtyping.

项目组通过探讨不同类型机会致病菌的种内遗传多样性情况及特定克隆的数据，讨论了病原菌分型诊断的重要性,为感染源和传染源的流行病学和流行病调查打开一个崭新的视野。以活动期肺结核患者全血及健康人全血为研究对象，初步建立了活动期肺结核患者全血中异常的转录因子-microRNA共调控网络，这一网络将有助于我们进一步了解肺结核病免疫应答的分子机制，并对未来肺结核病生物标志物和治疗靶点的研究提供新视角。通过中俄双方的学术互访，对病原菌种内分型及基因组条形码技术在真菌种内分子分型的应用进行了深入的讨论，并达成了进一步的合作。通过参加国际学术会议，向俄方介绍了本课题组的基因组条形码技术及其在细菌、病毒分型中的应用，展示了手足口病相关CVA16的分子进化研究工作，了解并学习到了俄方病原真菌种内分型的方法。