人工关节假体与骨质疏松骨的有效整合仍然是个难题。促进假体与骨质疏松骨的整合，对增强假体的稳定性，延长骨质疏松患者假体使用寿命有重要意义。西药副作用大，价格高，临床应用受到限制。骨碎补总黄酮能促进成骨细胞增殖、分化，促进骨髓间充质干细胞增殖和向成骨细胞分化。梯度二氧化钛纳米管有促进成骨作用，还可以作为药物载体形成缓释系统。本项目选用梯度二氧化钛纳米管作为骨碎补总黄酮的载体，在钛合金假体表面构建骨碎补总黄酮控释系统。从假体与骨质疏松骨整合的骨组织形态计量学、生物力学测试、Micro-CT等动物实验，成骨细胞和骨髓间充质干细胞增殖和粘附，成骨相关基因和蛋白的表达等多层面研究载骨碎补总黄酮梯度二氧化钛纳米管涂层对钛合金假体与骨质疏松骨整合的影响及机制，为该假体的研制和临床应用提供理论依据。

The effective integration of the prosthesis and osteoporotic bone is still a problem. To promote the osseointegration is of great significance to enhance the stability of the prosthesis and it can prolong the life of the prosthesis in patients with osteoporosis. The side effects and high price of Western medicine limit its clinical application. Total flavonoids of Rhizoma Drynariae can promote the proliferation and differentiation of osteoblasts and bone marrow mesenchymal stem cell. The gradient TiO2 nanotubes can promote osteogenesis. It also can be used as drug carriers to form drug delivery system. The project selects the gradient TiO2 nanotubes coating on titanium alloy prosthesis surface as the carrier of total flavonoids of Rhizome Drynariae, forming drug controlled release system. The titanium alloy implant osseointegration in osteoporotic rats are evaluated by the bone histomorphometry, biomechanical test and micro-CT. The proliferation and adhesion of osteoblasts and bone marrow mesenchymal stem cell, Osteogenic related genes and protein expression levels are measured to explore the mechanism of promoting osseointegration by gradient TiO2 nanotubes coating with total flavonoids of Rhizoma Drynariae on titanium alloy prosthesis. The study will provide a theoretical basis for the development and clinical application of the prosthesis.