中文摘要
香梨园苹果蠹蛾、梨小食心虫与香梨优斑螟同域混合发生,均钻蛀隐蔽为害,抗逆性强,致使化学农药使用频繁而防效低下,已严重制约新疆特色香梨产业发展。本项目以三种食心虫为害香梨时既竞争又共存的生态关系为切入点,从化学生态学角度研究三种害虫与共同寄主间的化学信息联系及虫害诱导挥发物介导的种内种间互作机制。拟采用动态顶空集气法收集健康香梨、机械损伤香梨、分别被三种害虫取食后的香梨挥发物,用GC-MS技术分析其成分与含量,并比较不同处理香梨挥发物组分质和量的差异;进而通过EAG、GC-EAD、风洞等技术测定三种食心虫对香梨自然挥发物和虫害诱导挥发物的电生理和行为反应,明确活性组分,并将其复配后在田间进行生物活性测定,筛选具有高效引诱或驱避作用的化学配伍。该研究不仅有助于在理论上理解植食性昆虫的寄主选择行为和同资源种团内多个竞争物种共存的化学生态机理,且有助于在实践中发展害虫行为生态调控的新途径和新技术。
英文摘要
Cydia pomonella, Euzophera pyriella and Grapholitha molesta are important fruit borers of fragrant pear orchard in South Xinjiang region, which have the latent threat to fragrant pear industry on account of some adaptable characteristics such as hidden habitat, boring damage, strong stress resistance to adversity and mixing co-occurrence on the same host plants. Chemical pesticides is frequent, but the control effect is low. It is particularly urgent and important to explore their new control approaches.In this study, Based on facts of significant intra- and inter-specific competition and coexistence relationship for three fruit borers, the mechanism of chemical information communication between three major fruit-boring herbivores and their same host plants and the role of herbivore induced plant volatile-mediated herbivore-herbivore intra- and inter-specific interactions are explored from the point of view of chemical ecology. For understanding the reaction mechanism of herbivore-induced fragrant pear volatiles in host plant orientation and selection of three herbivores, we take healthy, mechanically damaged host plants, and plants infested with larvae of the three fruit borers as research objects and obtain volatiles by using a dynamic headspace method. In order to make clear the ecological functions of fragrant pear volatiles, the volatile components are analyzed and identified by GC-MS, and the electroantennogram bioactivities and behavior responses of adults and larvae of three fruit borers to host Plant volatiles are tested by EAG or GC-EAD and "Y" tube olfactometry or wind tunnel, the active components are identified. On this basis, through the active components mixing and trap effect test in field, chemical compatibility of strong attracting or repelling effects are determined. The results of this research program are not only helpful to understand the host selection behavior and chemical-ecological mechanism of coexistence of multiple competition species of the same guild but also provide new pathways and innovative technologies of ecological regulation strategy on fruit borers in practice.
