NADPH氧化酶是植物质膜氧化还原系统的主要酶类之一, 在质膜活性氧的产生中起重要作用；植物MAP激酶级联途径则是将胞外刺激传递到胞内响应的重要途径，广泛参与氧爆发的信号过程。研究表明，NADPH氧化酶活性受到翻译后的调节，在ABA以及病原体侵染等条件下，MAP激酶级联途径参与NADPH氧化酶的激活及翻译后调节。本项目以超强抗寒油菜陇油6号和典型抗旱植物柠条为研究材料，克隆NADPH氧化酶基因，通过研究MAPKK抑制剂、H2O2清除剂、H2O2预处理和未经预处理，再经非生物胁迫处理后，MAP激酶信号转导途径相关基因的转录、H2O2产生与NADPH氧化酶基因转录活化水平变化及相关性分析，探讨在非生物胁迫下，MAP激酶信号转导途径、H2O2产生及NADPH氧化酶三者之间的交互作用和调控关系，为植物响应非生物胁迫的调节机制以及非生物胁迫信号在植物体内的传导过程提供新的参考依据。

NADPH oxidase is one of the main enzymes of plant plasma membrane redox system, which plays an important role in the generation of reactive oxygen species in the plasma membrane; MAP kinase cascades are important signaling modules that convert signals generated from the receptors/sensors to cellular responses, and broad participates in the signal process of oxygen outbreak. The NADPH oxidase activity is regulated post-translational. Studies have shown that under the treatment of ABA and pathogen infection, MAP kinase cascades is involved in the activation of NADPH oxidase and post-translational regulation. The project uses super cold-resistant Brassica campestris longyou 6 and typical drought-resistant plants Caragana korshinskii as research material, cloning NADPH oxidase gene, by studying MAPKK inhibitors, H2O2 scavenger, H2O2 pretreatment and without pretreatment, then after abiotic stress treatment, MAP kinase signal transduction pathway associated gene transcription, H2O2 generation and activation change of NADPH oxidase and correlation analysis, discussing under abiotic stress, MAP kinase signal transduction pathways, H2O2 generation, NADPH oxidase interaction and regulation relationship, the result provide new reference in plant response to abiotic stress and abiotic stress regulatory mechanisms of signal transduction processes in plants.