稻瘟病是世界各水稻产区危害极大的真菌性病害，稻瘟菌与水稻的互作已成为研究真菌病害的模式系统。在真菌生长发育和侵染寄主的过程中，如何适应pH的变化非常重要。目前关于真菌pH信号系统的研究主要集中于响应碱性pH信号的PacC信号途径，而其它pH信号途径的研究和报道却非常少，且缺乏系统性的研究。在前期的工作中，本课题组筛选获得了36个酸性pH敏感型ATMT突变体，并获得了所有突变体的突变位点信息。在本项目中，申请人将对这些突变体涉及的基因进行敲除验证，克隆一系列响应酸性pH的新基因，并从中鉴定出响应酸性pH的关键信号途径，最终揭示酸性pH信号途径在生长发育和致病过程中的作用机制。本项目研究将能为深入理解病原真菌致病机理提供新的思路，并可能为研发广谱性杀菌剂提供新的靶标。

Rice blast is a devastating fungal disease on rice throughout the world and is a model system for studying fungus-plant interactions. It is very important for the fungi that how they can adapt to pH changes during development and infection processes. So far, fungal pH regulation system studies are mainly focused on the alkaline pH-responsive PacC signaling pathway, but less refer to other pH systems, and lack of systematic research. In the preliminary works, we obtained thirty-six acidic pH-sensitive mutants and determined their T-DNA insertion sites. In this study, we plan to deletion and confirm all the putative acidic pH-responsive genes generated from those acidic pH-sensitive mutants. Then, we will identify key signaling pathways involving in acidic pH response and determine their roles during development and infection processes. Our study will provide novel views to understand fungal infection mechanism, and will also provide new target for developing broad-spectrum fungicides.