缺铁是限制苹果产业发展的重要因素之一，但迄今对调控苹果缺铁应答反应是否涉及铁区隔化调控尚不清楚。本项目以铁高效基因型小金海棠为试材，拟从缺铁胁迫下铁‘区隔化’存在的状态及其控制因素的角度，揭示可能控制缺铁应答反应的重要再分配机制。研究内容包括三个主要部分：第一部分“铁区隔化及缺铁应答关键铁载体筛选研究”，旨在揭示缺铁应答反应是否受铁的区隔化或再分配的调控，且筛选出调控过程中关键的铁载体基因；第二部分“候选基因的功能验证及其作用机理解析”，旨在最终确定操纵铁再分配的关键基因如何响应缺铁胁迫，及揭示铁再分配是否操纵植物抗缺铁胁迫的能力。第三部分“‘铁载体关键基因’的上游调控途径探究”，旨在通过鉴定该基因上游转录调控元件及相应转录因子，明确其转录调节机制。本研究不仅有助于深入了解苹果抗缺铁的分子机理，同时对苹果抗缺铁育种或分子改良具有重要意义。

Iron deficiency is one of key factors that limit the development of apple industry. So far, it remains unclear whether Fe compartmentalization regulate Fe-deficiency response. In this study, we use Fe-efficient genotype Malus xiaojinese as the material, to reveal the important mechanism that how iron redistribution regulate Fe-deficiency responses. This research proposal contains three major parts: studying on filter the key siderophores about Fe compartmentalization and Fe-deficiency responses, the first part is the basic molecular study on whether iron compartmentalization or redistribution involve in the Fe-deficiency responses, and screen the key iron transporters. The second part is on functional verification and characteristic of candidate genes to determination how the key genes respond to Fe-deficiency stress, and reveal whether iron redistribution has a role in Fe-deficiency resistance in planta. Finally, to uncover the transcriptional regulation mechanism by identification on relevant transcriptional factors of the key genes. In this study, not only have a further contribution to understand molecular mechanism in apple Fe-deficiency-resistance, but also have an important significance on Fe-deficiency-resistance breeding or molecular manipulation.