与苹果、梨等类似，草莓果实主要由花托而非子房发育而成，属于假果。生长素在果实发育过程中起着至关重要的作用，但我们对在假果实发育过程中生长素的合成起源与转运的时空调控机制还知之甚少。在前期二倍体草莓果实发育精细转录组分析基础上，通过qRT-PCR、启动子活性分析和RNA原位杂交等手段，本项目将详细解析生长素合成酶TAA和YUC家族及转运蛋白PIN家族在草莓果实中的表达部位和表达水平；结合生长素响应启动子DR5的转录活性和PIN蛋白在细胞膜上的极性分布，探察生长素的合成部位以及从合成部位转运到果皮和花托的运输路线和与发育时期的关系；通过酵母单杂交筛选调控生长素合成的转录因子。这些结果将有助于揭示假果类果实发育过程中生长素合成和转运的时空调控机制，为果树作物的遗传改良提供理论基础。

Similar to apple and pear, the edible flesh of strawberry fruit is derived from the receptacle rather than ovary, named false fruit. Auxin has fundamental roles in fruit development, but we don’t know where and when auxin is exactly synthesized and how it is transported in false fruit. On the basis of previous elaborate fruit transcriptome analysis in diploid strawberry, this project will carefully dissect the spatiotemporal expression of auxin biosynthetic gene TAAs and YUCs and transport gene PINs in the achene and receptacle of strawberry by qRT-PCR, promoter activity analysis and RNA in situ hybridization; combined with the activity of auxin responsive promoter DR5 and the polar distribution of PIN proteins in the plasma membrane, predict the tissues or cell types producing auxin and the transport path of auxin from the sources to the pericarp and receptacle, respectively; screen for transcription factors regulating the auxin biosynthesis by yeast-one-hybrid. The results will help to uncover the spatiotemparol regulation mechanisms of auxin biosynthesis and transport in the development of false fruit, providing theoretical foundations for genetic improvement of fruit crops.