微小牛蜱对新疆地区两大支柱产业-奶牛业和养羊业危害严重，有效防制微小牛蜱及其蜱传病已成为迫切需要解决的问题。免疫防制具有很强的应用前景，已有研究表明蛋白与DNA疫苗联合免疫可增强免疫效果，而对微小牛蜱双基因重组蛋白和DNA疫苗联合免疫效果及机制研究尚属空白。本项目在前期已人工饲养微小牛蜱和获得微小牛蜱免疫原性较好的2个基因Bm86和Bm91的基础上，拟利用共表达的优势，构建微小牛蜱Bm86和Bm91双基因DNA疫苗，同时进行原核共表达Bm86和Bm91重组蛋白，将构建的双基因DNA核酸疫苗和双基因重组蛋白疫苗联合免疫实验动物。通过测定体液免疫、细胞免疫、免疫保护率以及对微小牛蜱的损伤等指标与对照组的差异，进而对联合免疫效果进行评价并阐明其免疫机制。对研制新型抗微小牛蜱免疫制剂并有效预防和控制微小牛蜱及其蜱传病具有重要的理论意义与实际价值，为其他蜱及寄生虫免疫防制新策略提供理论与实验依据。

Boophilus microplus causes serious harm to pillar industries of cattle industry and sheep farming in Xinjiang. It is an urgent problem to prevent Boophilus microplus and its borne diseases effectively. The immune prevention method shows great application prospect and other studies have shown that combined immunization of protein and DNA vaccine can enhance the immune effects. However, the research of combining immune effect and mechanism of recombinant protein and DNA vaccine control Boophilus microplus is unknown. Thus further research is needed. In order to construct Bm86 and Bm91 digenic DNA vaccine and co-expresse digenic recombinant protein, Bm86 and Bm91 which have high immunogenicity antigen genes of Boophilus microplus were selected and obtained to take the advantage of common expression under the basis of breeded Boophilus microplusby the laboratory. Experimental animals will be vaccinated with digenic DNA vaccine and recombinant protein. The differences in cellular immunity, humoral immunity, immune protection ratio and damage of Boophilus microplus with control groups would be applied to evaluate combined immune efficacy and elucidate combined immune mechanism. Consequently, the study has theoretical significance and practical value for development of new immune preparation that could effectively prevent and control Boophilus microplus and its borne diseases, moreover, it may provide theoretic and experimental basis to new immune prevention strategies of other ticks and parasites.