颅内动脉瘤是严重危害人类健康的主要疾病之一。由于破裂后致死率和致残率都很高，对患者、家庭、社会造成沉重的压力。既往研究已证实miRNA在颅内动脉瘤的发生发展及破裂中具有重要作用。本课题组前期的预实验表明血清miRNA21的表达存在显著差异。本项目拟通过检测和比较颅内动脉瘤患者异常表达的miRNA21，结合其相应的靶基因等，探讨miRNA21在颅内动脉瘤中的作用及其机制，探寻与颅内动脉瘤破裂易感性生物标记，以期建立一种便宜、简单易行的颅内动脉瘤风险评估体系，识别高危人群，为早期发现可能破裂的颅内动脉瘤提供线索，这无疑对颅内动脉瘤的预防和治疗具有重大意义

Intracranial aneurysm is one of the major diseases which harm to human health seriously. Because of the high death rate, the high disability rate after the Aneurysm rupture, so it can make heavy pressure on patients, families and society. It has been proved that miRNA plays a significant role in the form, develop and rupture of intracranial aneurysm. Preliminary experiment result shows, the expression of miRNA21 of rupture group is significant different from contrast group. We plan to detect the abnormal expression of miRNA-21 in the intracranial aneurysm different group and explore its corresponding target gene. We hope to probe into the alarming effect and its mechanism of the miRNA21 which we hypothesis as the sensitive biomarkes of intracranial aneurysms, resultantly enables us to find a simple and reasonable system to value the risk of the aneurysm and segregate the highly vulnerable people in order to find out the clues of the rupture of the intracranial aneurysm in early stage. It is undoubtedly significant for the prevention and treatment of the intracranial aneurysms.

目的：通过基因芯片筛查探讨血清miRNA21在颅内动脉瘤破裂的预警作用方法： 24例在我科560例患者随机分为4组（n = 6）即造影阴性对照组，低危未破裂组，高危未破裂组（带子瘤），破裂组，根据实验要求,提取血液标本血清，然后进行了血清miRNA基因芯片筛查研究，通过测定实验组和对照组之间血清miRNA水平的差异，结果的主成分分析（PCA）和miRNA21差异表达进行了鉴定。聚类分析是为了使结果看起来更直观的和潜在的miRNA靶标预测数据库检索基因的目标结果：基因芯片的研究表面共有86个miRNA(包括miRNA21)在三个实验组和对照组之间存在差异显著（P＜0.05）的表达。其中69个上调表达，17下调表达。在A组所有的miRNA都上调表达，而一些上调和全部下调表达主要出现在B组和C组。共有8291个预测靶基因调节这些相关miRNAs。生物信息学分析显示，多个靶基因参与细胞凋亡和血管壁功能相关的细胞激活。结论：我们通过基因芯片的方法揭示了动脉瘤患者和正常人之间，以及动脉瘤的不同阶段之间，存在一些不同的血清miRNA21的表达，表明miRNA21可能参与颅内动脉瘤的发生、发展规律和颅内动脉瘤破裂有报警功能。差异表达的miRNA21在A组上调，结合其相应的靶基因等，发现miRNA21与miRNA106共同调控STAT3通路，参与血管的再生的关键环节，这为探寻与颅内动脉瘤破裂易感性生物标记，建立一种便宜、简单易行的颅内动脉瘤风险评估体系，识别高危人群，为早期发现可能破裂的颅内动脉瘤提供了很好的线索，无疑对颅内动脉瘤的预防和治疗具有重大意义