羊肚菌是一种具有很高经济价值的美味食用菌，但它的遗传背景较为复杂，菌丝和子囊孢子都为多核体，很难从单孢分离物中区分同核体和异核体，交配机制也不明确，这些都给规模化人工栽培带来较大难题。本项目利用前期工作中获得的红褐羊肚菌(Morchella rufobrunnea)转录组序列信息设计引物，DNA walking全长克隆羊肚菌三大类群中五个种的交配基因MAT1-1、MAT1-2和信息素a因子、alpha因子受体基因，以这四个基因为分子标记，把其中三个种中得到的单孢分离群体划分为同核体和异核体；区分后的同核体、异核体之间两两对峙培养，荧光原位杂交检测对峙拮抗菌丝中交配基因和信息素受体基因的表达情况，分析它们是否在交配时选择性表达，再结合子囊发育过程中核相变化的显微观察，从分子水平上探索羊肚菌的交配机制，为羊肚菌规模化人工栽培奠定坚实基础。

Morchella fungi are well-known and economically important edible mushroom, but it is difficult to artificially cultivate in large-scale for their complex genetic background and ambiguous mating mechanism. There are many nuclei in the mycelia and ascospores , which makes it hard to distinguish between homokaryon and heterokaryon in the single spore isolates. In this plane, the complete gene sequences of mating factor MAT1-1,MAT1-2 and pheromone receptor a,alpha in five species of Morchella genus will be obtained by DNA Walking based on the conserved segments from the transcriptome sequences of M.rufobrunnea in the previous study. The homokaryon and heterokaryon can be distinguished by amplified these four gene segments as the molecular marker in the single spore isolates of other three species. The expression leve of the genes of mating factor and pheromone receptor in the mating strains were determined by the method of fluorescence in situ hybridization in order to analyze whether they were selective expressed in mating , combined with observation of the nuclei change in the process of ascus development for exploring the mating mechanism in the molecular level and laying the foundation for the artificial cultivation of the Morchella in large-scale.