慢性下腰痛机制不明给临床诊治造成诸多困扰，椎间盘的“生物学改变”是引发腰椎退变的重要病理基础，干细胞移植有望解决这一问题，然而移植后的存活率和转化率有待提高。富血小板血浆的应用开启了全新的“自体生物学”治疗模式，并有望解决干细胞移植中的诸多难点。本课题组前期研究发现，椎间盘退变可能与Wnt/β-catenin 通路的异常激活有关，而补肾活血方与富血小板血浆联合应用，很可能调节该通路而影响干细胞的活性和分化、提高其在椎间盘内移植后的存活率和转化率，最终实现延缓椎间盘退变的作用，此类研究尚未见报道。.研究采用体外和体内两种模型，分别以人脂肪干细胞/髓核细胞共培养体系和兔腰椎间盘退变模型为对象，应用细胞培养、免疫化学、病理组织学及分子生物学检测技术，观察补肾活血方、富血小板血浆对干细胞移植治疗椎间盘退变的影响以及作用机制，从分子水平、细胞水平和整体动物水平探讨中医药防治椎间盘退变的机理。

Chronic low back pain could be caused by extremely complex etiological factors, which makes it difficult to be diagnosed and cured. Biochemical changes in intervertebral discs were considered to be the most important pathological basis. Stem cell transplantation might be an ideal method, while the survival and transformation rates of cells still remains to be improved. The applications of platelet-rich plasma opened up a door of “autologous biological treatment”. Based on the previous work, we conjecture that the combination of Kidney-tonifying and Blood Circulation-promoting Recipes and platelet-rich plasma could improve the survival and transformation rates of stem cells and delay intervertebral disc degeneration through Wnt/beta-catenin signaling pathway.. A rabbit model of disc degeneration and cells co-culture system were developed for the in vivo and in vitro experiments in the current research. By using multiple advanced medical technologies of cell culture, immunohistochemistry, histopathology and molecular biology, we evaluated the effect of Kidney-tonifying and Blood Circulation-promoting Recipes and platelet-rich plasma on stem cell transplantation. In addition, the Wnt pathway was observed to find out the molecular mechanism. The final results were expected to provide some scientific evidence for the research of TCM on the remediation of chronic degenerative bone and joint disease.