腹泻型肠易激综合征是多发病、难治病之一，其中医证候研究是中医辨证论治取得有效性的关键，本研究在前期分子生物学及临床研究的基础上，采用宏基因组学技术对中药（痛泻要方、参苓白术散）干预腹泻型肠易激综合征中医证候（肝郁脾虚证、脾虚湿阻证）前后的肠道微生态学变化进行研究，旨在揭示腹泻型肠易激综合征不同证型的宏基因组学差异、中医药辨证论治取得效果的宏基因组学依据以及获得各证型相关信号通路及关键基因的表达，达到以方测证，方证互验，从而为腹泻型肠易激综合征中医微观辩证奠定初步的肠道微生态宏基因组学基础。

IBS-D is a common and difficult to treat， study Syndromes of TCM is the key to the effectiveness ,This study is based on the prophase molecular biology and clinical research, using metagenomic technology of traditional Chinese medicine (Tong xie yao fang, Shen ling bai zhu san ) intervention IBS-D of TCM syndrome (liver depression and spleen vacuity syndrome, spleen vacuity and damp encumbrance syndrome) were studied before and after the change of intestinal microecology, to reveal the IBS-D in different syndromes of metagenomics differences in traditional Chinese medicine based on syndrome differentiation achieved the effect of metagenomic basis and expression various types of related signaling pathways and key genes, designed to reveal the IBS-D with different syndromes metagenomic differences, the Chinese medicine diagnosis and treatment to be effective metagenomic basis to obtain each permit differential metagenomic expression，a preliminary metagenomic basis for IBS-D micro- syndrome differentiation