周围神经损伤是临床常见创伤，许多病例致伤后终身残疾。临床常用的修复神经的方法包括神经原位缝合、神经移植、神经套管等。除此之外，还有很多方法已被实验证实可以有效促进神经再生。然而，尽管形态学上观察到神经组织的再生，但患肢功能恢复并不理想，特别是神经大段缺损，肢体功能可能几近丧失。其中一个重要原因就是再生的神经与远端靶器官不能实现有效的对接。神经损伤后，远端断端发生华勒变性，神经肌肉接头发生结构和功能的改变。随着失神经支配时间的延长，突触后膜的乙酰胆碱受体亚单位也不再局限于突触后膜，而是广泛分布于肌纤维；运动终板数量减少，以至于最终消失。神经再生能否成功最终取决于神经肌肉接头能否有效重建。以往的神经再生的研究多数只关注神经组织的再生情况，但对于神经肌肉接头的恢复了解甚少。本研究旨在通过对神经肌肉接头的观察，了解课题组前期探索出的多种神经损伤创新修复方法的最终效果，以期更好地改善肢体功能恢复。

Peripheral nerve injury is a common clinical traumatical disease, which at most time results in disability. The commonly used nerve repair approaches include nerve autograft, bridging conduit and so on. In addition, many other approaches have been identified to be effective to promote nerve regeneration. However, even though the nerve regeneration was observed morphologically, the injuried extremity was not recovered ideally, especially for the long defect, the function of extremities were nearly lost. One of the most important reasons is that the regenerated nerve can not form effective link with the objective organism. After nerve injury, Wallerian degeneration happened in the distal nerve stump, and structural and functional changes happened in the neuromuscular junction.With the elongation of denervation, the acetylcholine receptor subunits were not limited on the postsynaptic membrane but widely distributed in the fibers. The number of endplate decreased and at last diminished. Success of nerve regeneration depends on the successful reconstruct of neuromuscular junction. The previous study on nerve regeneration mostly focused on the regeneration of nerve, but little was known about the neuromuscular junction recovery. This study aims to learn the final effect of novel approaches of nerve repair that were discovered previously by our lab, with the purpose of improve the recovery of extremity function.

周围神经损伤是临床常见创伤,临床常用的修复神经的方法包括神经原位缝合、神经移植、神经套管等。除此之外,还有很多方法已被实验证实可以有效促进神经再生。然而,尽管形态学上观察到神经组织的再生,但患肢功能恢复并不理想，特别是神经大段缺损,肢体功能可能几近丧失。其中一个重要原因就是再生的神经与远端靶器官不能实现有效的对接。神经损伤后,远端断端发生华勒变性,神经肌肉接头发生结构和功能的改变。随着失神经支配时间的延长,突触后膜的乙酰胆碱受体亚单位也不再局限于突触后膜,而是广泛分布于肌纤维;运动终板数量减少,以至于最终消失。神经再生能否成功最终取决于神经肌肉接头能否有效重建。本研究旨在通过对神经肌肉接头的观察,了解课题组前期探索出的多种神经损伤创新修复方法的最终效果,以期更好地改善肢体功能恢复。本研究按计划进展顺利，本年度发表1篇SCI文章，另外有4篇英文文章已经完成撰写并投稿于SCI国际期刊。