本项研究立足于解决高通量细胞过滤分离中的细胞堵塞问题。通过细致地对前期高通量细胞过滤研究中发现的细胞堵塞现象开展理论分析，申请人发现过孔过程总细胞之间的相互作用所导致的局部高细胞浓度是堵塞发生的一个主要诱因。基于此假设，通过中韩双方团队的前期讨论，拟应用超声激励来减小细胞相互之间的粘附作用，并力图通过可设计的声力来减缓细胞过孔速度，使得近滤孔区局部细胞浓度降低。.合作双边在本项目中的职责如下：.-中方：制备微孔阵列滤膜；设计并组装过滤系统；组装超声元件与过滤系统；设计并开展细胞过滤实验验证超声辅助的细胞过滤效果。.-韩方：研究满足项目需求的超声条件（强度、频率等），以在保持细胞活性的前提下避免细胞堵塞；设计并选择适宜的超声元件；评价超声元件的工作特性；理论研究超声与细胞的相互作用。

The research is targeting at solving the clogging issue existed in the high-throughput cell filtration. After a careful theoretical analysis of the cell clogging phenomena existed in the previous high-throughput cell filtration, we found the adhesion between cells when they pass through the filtration pore at a high local concentration might be the main reason for the cell clogging. Based on this hypothesis, an ultrasonic stimulation is proposed to reduce the adhesive interaction between cells, and a designable acoustic force generated by ultrasound will be used to repel the cells from the filtration membrane to reduce the local cell clogging. ..Duties of the two parts in this collaborative research:.-.Chinese part: Fabricating the filtration membrane; designing and preparing the filtration system; assembling the filtration system with the ultrasonic components; designing and carrying out the cell separation experiments to evaluate the filtration performance..-.Korean part: Finding out the ultrasound conditions (ultrasonic intensity, frequency, etc.) to avoid cell clogging while keeping the cells alive, designing or choosing proper ultrasonic transducers, evaluating the ultrasound characteristics, and theoretical analysis of the ultrasound field and interaction between ultrasound and cells.