地球上所有动物包括人类，其生物钟节律的紊乱是妨碍正常生命活动的主导因素之一，因此睡眠和活动节律目前是国际上研究的热点和焦点领域。保幼激素（Juvenile Hormone, JH）是昆虫体内最重要的内分泌激素之一，主要由咽侧体（Corpora Allata, CA）合成和分泌。并受到脑中枢的控制。昆虫在生长发育、蜕皮变态及生殖等过程中，保幼激素均发挥着非常重要的调控作用（Zhang et al., 2010）。在黑腹果蝇中，我们通过Gal4/UAS转基因技术调控CA细胞中靶标基因的表达，初步发现JH参与调控其睡眠和昼夜活动节律（见下面前期工作基础）。本项目将在此基础上详细研究JH对睡眠和昼夜节律的行为调控，以及JH与钟基因之间的互作关系，进而探索JH参与的生物钟节律的新的调控基因和作用机制。因此，该项目的实施为生物节律的认知提供重要的理论依据，对学科发展也具有重要的科学意义。

All animals including human on the earth, the rhythmic disorder of biological clock is one of primary factors for Interfering with the normal life activities. Therefore, the sleep and locomotor rhythm of animals are currently hot and focus area in the world. In insects, juvenile hormone (JH) is one of the most important endocrine hormones synthesized primarily in the Corpora Allata (CA) with neural connections to the brain, that play a central role in the control of many biological processes such as development and reproduction (Zhang et al., 2010). In Drosophila melanogaster, we utilitied the technique of the Gal4/UAS to regulate expressions of target gene in brain or CA, and found that JH involved in regulating sleep and circadian locomotor rhythm (Please see the work basis below). In this fundation, we will explore detailedly the regulative action of JH on sleep and circadian locomotor rhythm, and further work on correlations between JH and clock genes. This will supply important theoretical basis for cognition of biological rhythm in animals, and there is critical scientific meaning to the disciplinary development.