植物病毒需要寄主蛋白的帮助才能移动和侵染，明确与病毒移动相关的寄主蛋白有助于研发新的抗病毒策略。P3N-PIPO是马铃薯Y病毒属病毒中新发现的移动蛋白，对其互作蛋白的研究很少。烟草脉带花叶病毒（TVBMV）主要侵染茄科作物，近年在马铃薯、番茄等作物上的危害呈上升趋势，可侵染大多数马铃薯和番茄品种。TVBMV P3N-PIPO能与番茄和烟草的DREPP蛋白互作，但不能与马铃薯的DREPP蛋白互作；沉默DREPP基因可阻止TVBMV 在番茄和烟草中移动，但不能阻止其在马铃薯中的移动。本项目拟利用酵母双杂交、双分子荧光互补、免疫共沉淀技术鉴定与TVBMV P3N-PIPO互作的马铃薯蛋白，明确其表达动态、亚细胞定位、体内转运途径，阐明其在TVBMV及相关病毒移动过程中的作用。研究结果将为培育抗TVBMV马铃薯品种提供新的靶标，也能为可持续控制马铃薯Y病毒属其他病毒提供新的思路。

Plant viruses recruit host proteins to move and establish systemic infection. Mapping the plant proteins interacting with viral movement proteins will be helpful to explore new strategy for viral diseases control. P3N-PIPO is a newly discovered frameshift protein encoded in the genome of viruses from family Potyviridae and regulates the cell-to-cell movement of potyviruses. The function and mechanism of P3N-PIPO interacting host proteins remains largely unknown. Tobacco vein banding mosaic virus (TVBMV) is a distinct species of the genus Potyvirus and of increasing importance in potato production. By using yeast two-hybird system, BiFC and Co-IP assay, DREPPs from tomato and tobacco were identified to interact with TVBMV P3N-PIPO. Silencing the DREPP gene using virus-induced gene silencing (VIGS) blocked the movement of TVBMV in tomato and tobacco, but not in potato. In this research, we will identify the protein partner of potato interacting with TVBMV P3N-PIPO, elucidate its interaction domain and subcellular localization, and analyze the effects of P3N-PIPO interacting protein on plant development and movement of TVBMV. The expected results will provide novel targets for breeding potato cultivars resistant to TVBMV and clues for exploring novel strategies for the control of other potyviruses.