木质纤维素是自然界最丰富的可再生清洁资源，微生物降解是当前木质纤维素利用最环保且能耗最低的方法。然而，木质纤维素降解菌活力有限，降解机制不明确等限制了该资源的有效利用。本研究以前期从暗黑鳃金龟幼虫肠道中所构建的高活力的秸秆降解菌系为对象，运用现代分析手段，研究秸秆降解过程中表观、化学结构以及降解产物的时空变化规律，揭示秸秆降解的次序和途径；运用现代微生物学分子技术研究手段，研究秸秆降解过程中微生物群落结构的时空演替规律，揭示秸秆降解的微生物协同机制；运用酶学特性分析技术，研究秸秆降解过程中木质纤维素降解酶系的时空分泌规律，揭示秸秆降解的酶系协同机制；最后通过对秸秆降解产物、微生物和酶系组成等研究数据进行多因素耦合和相关性分析，构建秸秆高效降解模型，为高效木质纤维素降解菌以及相关酶系的筛选和应用提供理论依据，为秸秆资源高效利用工艺的开发及现有工艺的改良奠定理论和技术基础。

Lignocellulose is one of nature’s most abundant renewable resources. Nowadays, microbial degradation is the greenest and lowest energy consumption way for application of lignocelluloses. However, because of that many strains showed low degradation abilities, and lignocelluloses degradation mechanisms were not fully clear, which limit the efficient utilization of lignocellulose resources. Our study was focus on the rice straw degradation composite flora, which was isolated from the hindgut of Holotrichia parallela larvae. The apparent, chemical structures of rice straw and degradation products would be analysed to illustrate the degradation way of rice straw. And the microbial and enzyme compositions and diversities would be analysed to reveal the microbial and enzymic synergistic degradation mechanisms. Furthermore, multi-factor coupling and correlation analysis would be empolied to build a efficient degradation model of rice straw. In some extent, those study will not only provide theoretical and technological basis for the screening of the lignocellulolytic microorganisms and enzymes, but also for the development of straw utilization technology and the improvement of existing process.