证候之科学内涵的研究是深入理解"异病同治"和"同病异治"的关键。项目组前期对脂肪肝和慢性乙型肝炎两种肝脏疾病的湿热证进行了研究，并初步探讨了"异病同证"的生物学基础，发现湿热证不仅与人体代谢网络，而且还可能与菌群代谢及微量元素代谢相关。但该结果是否适用于其他系统疾病的湿热证，还有待进一步深入研究与验证。为此申请课题选择病位分别在上、中、下三焦的3种疾病（上呼吸道感染、慢性乙型肝炎和尿路感染）的湿热证患者为研究对象，并采用GC-MS技术分析血液和粪便样本，加之ICP-MS技术分析尿液样本，分别从人体代谢网络、肠道菌群代谢网络和微量元素代谢的视角，全面探讨湿热证的证候基础，并分别探求湿热证在该三种疾病中的共性标志物和特异性病位标志物。继而用"三仁汤"干预湿热证模型大鼠，考察大鼠体内整体代谢网络的变化，并结合临床实验结果，探讨湿热证生物标志物及标志物群，根据用药前后变化进一步探讨其病变机制。

The research on connotation of syndrome is the key piont to understand the "same treatment for different diseases"and "different treatment for the same disease". Our group previously chose two liver diseases to explore the syndrome basis of damp-heat syndrome. But whether the basis of dampness-heat syndrome is suitable for other system diseases still awaits for being researched. So now we select patients with upper respiratory tract infection, chronic hepatitis B and urinary tract infection,respectively,as our objects, which are towards triple energizers,to more deeply explore the connotation of Deap-heat sydrome. We try to explore the syndrome basis of damp-heat syndrome from the view point of human body metabolic network, intestinal flora metabolic network and trace elements, and illustrate the relationship between the syndrome basis and body metabolic. Then we will use "San Ren Tang" to intervene the "dampness-heat syndrome rat model". Based on the overall metabolic network in rats and clinical results, we will explore the disease mechanism of dampness-heat syndrome.

选择病位分别在上、中、下三焦的3种疾病（上呼吸道感染、慢乙肝和尿路感染）的湿热证患者为研究对象，并以上述病种非湿热证者和健康者为对照，采用气相色谱质谱连用技术（GC-MS）对样本进行小分子代谢产物的分析，用电感耦合等离子体质谱（ICP-MS）对样本进行微量元素的分析，探讨上中下三焦湿热证的共性物质基础和个性差异性物质基础，寻找湿热证的临床生物标志物，从整体代谢网络上探讨湿热证与机体代谢的关系。用已得到的生物标志物评价现有湿热证动物模型，筛选出最接近临床湿热证状态的动物模型。并用“三仁汤”对该模型大鼠进行干预，根据回归正常的生物标志物，探讨湿热证的病变机制。