NF-κB是一组真核细胞转录因子，通过调控众多靶基因的转录表达而在免疫、炎症、细胞增殖与凋亡及肿瘤发生等许多生理学过程中发挥重要作用。但该基因在与DNA损伤修复间的关系报道较少。本研究通过采用多种RNA干扰技术（质粒载体介导的稳定表达、质粒载体介导的瞬时表达、慢病毒载体介导的稳定表达、小RNA片段介导的瞬时表达）探寻高效的NF-κBp65缺陷细胞模型。以环境化学污染物（ECPs）中氧化损伤的毒物代表过氧化氢（H2O2）为暴露因素，研究NF-κBp65缺陷细胞在H2O2的不同暴露水平下DNA损伤，细胞凋亡情况，并检测不同暴露水平下DNA损伤修复的相关蛋白（P53, HSP70, Bcl-2, Casepase-3, PARP, 断裂的PARP）的表达，分析NF-κBp65在DNA损伤修复通路中的作用，为明确NF-κB在机体对外界刺激的应答反应中的作用提供线索。

NF-κB is a nuclear transcription factor that may activate the transcription of many target genes involved a wide variety of biological responses such as immune responses, inflammation, proliferation, apoptosis and oncogenesis. But few studies have focused on the relationship between NF-kB and DNA damage-repair. In this research, the model of NF-κBp65 knocked down was explored using different RNA interference technology. H2O2, a type of ECPs(environment chemical pollutions) and the representative of oxidative stress toxicants, was the exposure agent . The effect of NF-κBp65 knocked down on cells treated with the different H2O2 concentration by DNA damage, apoptosis, and the expression of some protein associated with DNA damage-repair(P53, HSP70, Bcl-2, Casepase-3, PARP, cleaved PARP), then analyzing the role of NF-κBp65 in the signaling pathways, Which was the clue to identify the NF-κB role in body defense response.

NF-κB是一组真核细胞转录因子，通过调控众多靶基因的转录表达而在免疫、炎症、细胞增殖与凋亡及肿瘤发生等许多生理学过程中发挥重要作用。但该基因在与DNA损伤修复间的关系报道较少。本研究通过采用多种RNA干扰技术（质粒载体介导的稳定表达、质粒载体介导的瞬时表达、慢病毒载体介导的稳定表达、小RNA片段介导的瞬时表达）探寻高效的NF-κBp65缺陷细胞模型。以环境化学污染物（ECPs）中氧化损伤的毒物代表过氧化氢（H2O2）为暴露因素，研究NF-κBp65缺陷细胞在H2O2的不同暴露水平下DNA损伤，细胞凋亡情况，并检测不同暴露水平下DNA损伤修复的相关蛋白（P53, HSP70, Bcl-2, Casepase-3, PARP, 断裂的PARP）的表达，分析NF-κBp65在DNA损伤修复通路中的作用，为明确NF-κB在机体对外界刺激的应答反应中的作用提供线索。