中文摘要
刺梨（Rosa roxburghii Tratt）依其极高Vc含量著称，但在人工栽培时白粉病（Sphaerotheca pannosa）为害严重。本课题利用抑制性差减杂交（SSH）技术及病原诱导后高抗株系（贵农6号）特异表达的cDNA 片段差减文库，深入开展刺梨白粉病高抗株系寄主防卫反应的分子机理研究，筛选出了高抗寄主增强表达基因片段；通过测序与GenBank 数据库检索，分析了这些片段与抗病反应的关系，发现光呼吸相关基因与抗病性密切相关，并对这些基因进行了结构预测；采用Real-time PCR检测接种白粉菌前后抗病相关基因的表达情况，并对这些基因编码的光呼吸相关酶类活性变化进行测定，进一步证实光呼吸基因在刺梨白粉病防卫反应中起着至关重要作用。通过RACE扩增，获得4个抗病相关基因的全长序列。课题还对刺梨RGA 基因表达, 蔷薇科RGA 基因的比较分析,刺梨与白粉菌的互作及寄主的生化反应等内容进行了探讨。课题发表论文7篇，获贵州省科技进步二等奖1项。
英文摘要
Powdery mildew （Sphaerotheca pannosa）of chestnut rose（Rosa roxburghii Tratt） is the most widely recognized and common disease of this plant. The defence molecular mechanism of high resistance host plant to this disease had been unraveled by Suppresion Subtrative Hybridization (SSH) strategy. The resistance-related gene analogs were cloned, and the useful information of isolated analogs was elucidated via sequence analysis. With real-time PCR technique, the expression levels of the interested genes were quantified before and after the inoculation of powdery mildew. Further, the enzyme activities translated from these genes were also measured. The obtained results had substantially verified that the photorespiration-related genes play highly roles in the defence response to powdery mildew of chestnut rose. Using RACE strategy, the full length of four resistance-related genes had been obtained herein. Additionally, the expession analysis of resistance gene analogs (RGAs), phylogenetic and evolutionary analysis of NBS-encoding genes in Rosaceae fruit crops, as well as biochemical responses to the powdery mildew fungi had been carried out in this crop. As a result, a total of seven papers, as well as a second prize of Science and Technology Advance Prize awarded by Guizhou Provincial Government were harvested based o
结题摘要
刺梨（Rosa roxburghii Tratt）依其极高Vc含量著称，但在人工栽培时白粉病（Sphaerotheca pannosa）为害严重。本课题利用抑制性差减杂交（SSH）技术及病原诱导后高抗株系（贵农6号）特异表达的cDNA 片段差减文库，深入开展刺梨白粉病高抗株系寄主防卫反应的分子机理研究，筛选出了高抗寄主增强表达基因片段；通过测序与GenBank 数据库检索，分析了这些片段与抗病反应的关系，发现光呼吸相关基因与抗病性密切相关，并对这些基因进行了结构预测；采用Real-time PCR检测接种白粉菌前后抗病相关基因的表达情况，并对这些基因编码的光呼吸相关酶类活性变化进行测定，进一步证实光呼吸基因在刺梨白粉病防卫反应中起着至关重要作用。通过RACE扩增，获得4个抗病相关基因的全长序列。课题还对刺梨RGA 基因表达, 蔷薇科RGA 基因的比较分析,刺梨与白粉菌的互作及寄主的生化反应等内容进行了探讨。课题发表论文7篇，获贵州省科技进步二等奖1项。